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Paper Information

Journal:   GENETICS IN THE 3RD MILLENNIUM   SPRING 2016 , Volume 14 , Number 2; Page(s) 4262 To 4269.
 
Paper: 

IDENTIFICATION OF SSR MARKERS ASSOCIATED WITH RESISTANCE TO POTATO VIRUS Y IN TOBACCO GERMPLASM

 
 
Author(s):  DARVISHZADEH REZA*, HEIDARI AHMAD, HATAMI MALEKI HAMID
 
* DEPARTMENT OF PLANT BREEDING AND BIOTECHNOLOGY, URMIA UNIVERSITY, URMIA, IRAN
 
Abstract: 

Potato Virus Y (PVY) is one of the most important pathogenic agents that reduce the yield and quality of tobacco (Nicotiana tabacum L.). DNA-based molecular markers are valuable tools for identifying germplasm in gene banks, assessing kinship ties, and doing marker-assisted selection (MAS) for resistance to pests, diseases, and some crop traits of interest. This work presents the results of association analysis for resistance to PVY with SSR markers. A total number of 66 alleles were detected using 26 SSR primer pairs on 90 oriental-type tobacco genotypes with an average of 2.54 alleles per locus. A model-based Bayesian approach was used to subdivide 90 tobacco genotypes into three subgroups. Approximately 5.85% of the 325 SSR locus pairs showed a significant level of linkage disequilibrium (LD) (P<0.01). By using GLM procedure, 3 loci (PT30260, PT30046 and PT30061) from linkage groups 8a, 6, and 11 of the tobacco reference map were identified to associate with resistance to PVY in a 10% probability level (P ? 0.0615, 0.0839, and 0.072, respectively). SSR locus PT30260 (P? 0.077) was also detected with the mixed linear model (MLM) accounting for population structure and kinship relatedness (Q+K model). Linked-resistant SSR markers can be used as indicators of PVY resistance and interest in marker-assisted selection (MAS) in tobacco breeding programs.

 
Keyword(s): GENETIC ASSOCIATION STUDIES, LINKAGE DISEQUILIBRIUM, MICROSATELLITE MARKERS, POTYVIRUS, TOBACCO
 
 
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Click to Cite.
APA: Copy

DARVISHZADEH, R., & HEIDARI, A., & HATAMI MALEKI, H. (2016). IDENTIFICATION OF SSR MARKERS ASSOCIATED WITH RESISTANCE TO POTATO VIRUS Y IN TOBACCO GERMPLASM. GENETICS IN THE 3RD MILLENNIUM, 14(2), 4262-4269. https://www.sid.ir/en/journal/ViewPaper.aspx?id=508682



Vancouver: Copy

DARVISHZADEH REZA, HEIDARI AHMAD, HATAMI MALEKI HAMID. IDENTIFICATION OF SSR MARKERS ASSOCIATED WITH RESISTANCE TO POTATO VIRUS Y IN TOBACCO GERMPLASM. GENETICS IN THE 3RD MILLENNIUM. 2016 [cited 2021May13];14(2):4262-4269. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=508682



IEEE: Copy

DARVISHZADEH, R., HEIDARI, A., HATAMI MALEKI, H., 2016. IDENTIFICATION OF SSR MARKERS ASSOCIATED WITH RESISTANCE TO POTATO VIRUS Y IN TOBACCO GERMPLASM. GENETICS IN THE 3RD MILLENNIUM, [online] 14(2), pp.4262-4269. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=508682.



 
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