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Paper Information

Journal:   GENETICS IN THE 3RD MILLENNIUM   SPRING 2016 , Volume 14 , Number 2; Page(s) 4250 To 4255.
 
Paper: 

CLONING AND EXPRESSION OF THE G1 EPITOPE OF BOVINE EPHEMERAL FEVER VIRUS G GLYCOPROTEIN IN ESCHERICHIA COLI

 
 
Author(s):  BEYGI NASSIRI MOHAMMAD TAGHI, PASANDIDEH REZA*, SEYFI ABAD SHAPOURI MASOUD REZA
 
* DEPARTMENT OF ANIMAL SCIENCE, FACULTY OF ANIMAL & FOOD SCIENCE, KHUZESTAN RAMIN AGRICULTURE AND NATURAL RESOURCES UNIVERSITY
 
Abstract: 

Bovine ephemeral fever (BEF) is an acute epidemic disease in cattle and water buffalo, spanning tropical and subtropical zones of Asia, Australia, and Africa. In recent years, BEF has been distributed in many provinces of Iran and caused economic losses. In this study the sequence encoding the G1 epitope of bovine ephemeral fever virus (BEFV) G glycoprotein was amplified by PCR, ligated into the prokaryotic expression vector, pMAL-c2X, and subsequently cloned into Rosetta strain of Escherichia coli. After being induced with Isopropylb-D-1-thiogalactopyranoside, SDS-PAGE analysis showed that a protein of 58 kDa molecular weight, consistent with the expected molecular weight of maltose binding protein-G1 fusion protein, was expressed.
Also, dot blot analysis confirmed that the expressed protein has specifically reacted with an anti-BEFV mouse serum. In conclusion, the results indicate that the recombinant G1 epitope expressed in this study could be used as a coating antigen to develop an ELISA for bovine ephemeral fever diagnosis.

 
Keyword(s): BOVINE EPHEMERAL FEVER VIRUS, ESCHERICHIA COLI, GENE EXPRESSION, G1 EPITOPE
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

BEYGI NASSIRI, M., & PASANDIDEH, R., & SEYFI ABAD SHAPOURI, M. (2016). CLONING AND EXPRESSION OF THE G1 EPITOPE OF BOVINE EPHEMERAL FEVER VIRUS G GLYCOPROTEIN IN ESCHERICHIA COLI. GENETICS IN THE 3RD MILLENNIUM, 14(2), 4250-4255. https://www.sid.ir/en/journal/ViewPaper.aspx?id=508680



Vancouver: Copy

BEYGI NASSIRI MOHAMMAD TAGHI, PASANDIDEH REZA, SEYFI ABAD SHAPOURI MASOUD REZA. CLONING AND EXPRESSION OF THE G1 EPITOPE OF BOVINE EPHEMERAL FEVER VIRUS G GLYCOPROTEIN IN ESCHERICHIA COLI. GENETICS IN THE 3RD MILLENNIUM. 2016 [cited 2021July31];14(2):4250-4255. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=508680



IEEE: Copy

BEYGI NASSIRI, M., PASANDIDEH, R., SEYFI ABAD SHAPOURI, M., 2016. CLONING AND EXPRESSION OF THE G1 EPITOPE OF BOVINE EPHEMERAL FEVER VIRUS G GLYCOPROTEIN IN ESCHERICHIA COLI. GENETICS IN THE 3RD MILLENNIUM, [online] 14(2), pp.4250-4255. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=508680.



 
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