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Paper Information

Journal:   IRANIAN JOURNAL OF PLANT BIOLOGY   FALL 2015 , Volume 7 , Number 25; Page(s) 69 To 84.
 
Paper: 

CALLOGENESIS IN ROOT EXPLANTS OF FOUR SPECIES OF THE FAMILY SOLANACEAE AFTER INDUCING BY AGROBACTERIUM RHIZOGENES

 
 
Author(s):  SHAKERAN ZAHRA, KEYHANFAR MEHRNAZ*
 
* DEPARTMENT OF BIOTECHNOLOGY, FACULTY OF ADVANCED SCIENCES AND TECHNOLOGIES, UNIVERSITY OF ISFAHAN, ISFAHAN, IRAN
 
Abstract: 

Studying explants affected by Agrobacterium rhizogenes shows that in addition to possible formation of hairy roots, it is likely that callogenesis can be induced in these tissues. The T-DNA region of A. rhizogenes codes enzymes that participate in biosynthesis of plants growth hormones. These hormones also affect callogenesis, hence, the formation of various calluses with different morphological properties are possible. It is very likely that the level of biosynthetic growth hormone, the plasmid carried by each bacteria strain, the position of T-DNA, and the level of gene expression contribute to this morphologic variation. In this study, the root explants of four species of the family Solanaceae namely Atropa belladonna, Datura metel, D. stramonium and Hyoscyamus niger were induced by using different strains of A. rhizogenes (A4, A7, AR15834, AR318, AR9402 and AR9543). Some of these explants entered callus phase and formed various calluses with different colors and shapes. Moreover, in some callus samples hairy roots were also appeared. These variations were probably caused by variations in the levels and ratios of auxin and cytokinine hormons after the induction. As shown in previous studies, the amount of secondary metabolites is reduced due to undifferentiated tissue produced in the callogenesis process.

 
Keyword(s): AGROBACTERIUM RHIZOGENES, SOLANACEAE, CALLOGENESIS, SECONDARY METABOLITES, T-DNA
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

SHAKERAN, Z., & KEYHANFAR, M. (2015). CALLOGENESIS IN ROOT EXPLANTS OF FOUR SPECIES OF THE FAMILY SOLANACEAE AFTER INDUCING BY AGROBACTERIUM RHIZOGENES. IRANIAN JOURNAL OF PLANT BIOLOGY, 7(25), 69-84. https://www.sid.ir/en/journal/ViewPaper.aspx?id=508258



Vancouver: Copy

SHAKERAN ZAHRA, KEYHANFAR MEHRNAZ. CALLOGENESIS IN ROOT EXPLANTS OF FOUR SPECIES OF THE FAMILY SOLANACEAE AFTER INDUCING BY AGROBACTERIUM RHIZOGENES. IRANIAN JOURNAL OF PLANT BIOLOGY. 2015 [cited 2021July26];7(25):69-84. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=508258



IEEE: Copy

SHAKERAN, Z., KEYHANFAR, M., 2015. CALLOGENESIS IN ROOT EXPLANTS OF FOUR SPECIES OF THE FAMILY SOLANACEAE AFTER INDUCING BY AGROBACTERIUM RHIZOGENES. IRANIAN JOURNAL OF PLANT BIOLOGY, [online] 7(25), pp.69-84. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=508258.



 
 
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