Paper Information

Journal:   IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE   FALL 2015 , Volume 20 , Number 70; Page(s) 33 To 41.
 
Paper: 

POLYMORPHISM IN THE BORDETELLA PERTUSSIS VIRULENCE FACTORS PERTACTIN AND FIMBRIAE IN IRAN

 
 
Author(s):  JANNESAR AHMADI NAZANIN, HOSSEINPOUR MARYAM, NIKBIN VAJIHE SADAT, NAKHOST LOTFI MASOUMEH, SHAHCHERAGHI FERESHTEH*
 
* 
 
Abstract: 

Background and objective: whooping cough is an acute and contagious infection that caused by Bordetella pertussis, a gram negative and human-specific pathogen. In the last decade, despite high vaccination coverage against pertussis in developed and developing countries, pertussis has reemerged in many countries including Iran.
Among several reason of resurgence of pertussis, one of the most important factors in outbreak of the disease might be polymorphism and genetic variation of virulence genes of bacteria. pertactin is considered as one of the important virulence factors in B.pertussis and Polymorphism in pertactin is essentially limited to region 1. Fimbriae or pillus antigens of B. pertussis have been shown to be one of the many adhesions present on the surface of the bacteria.
The purpose of this research was to study polymorphism of region 1 of prn gene and fim3 gene in circulating isolates and compare with vaccine strains.
Materials and methods: We examined 35 isolates isolated from nasopharyngeal specimen collected in 2008-2012.These strains have also been identified by biochemical and slide agglutination tests. Region 1 of prn gene and the fim3 gene in these isolates of B. pertussis were amplified by using specific primers by PCR method. Then we sequenced genes and analyzed our results by chromas and mega4 software. We examined also vaccine strain (134) .
Result: Our results showed that all examined strains have prn and fim3 gene in their genome in size 600 bp and 800 bp, respectively, as a partial codon. Also 34 strains have prn 2 and fim3-2 alleles and only one strain has prn 1 and fim3-1 allele. strain 134 showed prn 1 and fim 3-1 alleles.
Conclusion: In studies that have been done in most European and American countries with high vaccination coverage, similar results have been seen and predominant alleles were prn2 and fim3-2. We also obtained prn2 and fim3-2 as a predominant allele in our result. This study is performed for the first time in Iran. Investigation in the world suggest that vaccination has selected for strains which are antigenically distinct from vaccine strains, also considering the importance of polymorphism genes encoding virulence and immunogenic factors of B. pertussis, it's very important to use the clinical strain that has dominant alleles of these virulence factors for production of effective vaccine against pertussis.
Consequently, it seems that more research is needed on the polymorphism of other virulence factors of B. pertussis in circulating strains in our country.

 
Keyword(s): POLYMORPHISM, PERTACTIN, FIMBRIAE
 
References: 
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