Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)   2016 , Volume 17 , Number 2 (55); Page(s) 130 To 133.
 
Paper: 

SHORT PAPER: FIRST REPORT OF MIRU-VNTR GENOTYPING OF MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS ISOLATES FROM EGYPT

 
 
Author(s):  FAWZY A.*, FAYED A., YOUSSEF H., EL SAYED A., ZSCHOCK M.
 
* HESSIAN STATE LABORATORY (LHL), GIESSEN, GERMANY
 
Abstract: 

Mycobacterium aviumsubsp. paratuberculosis (MAP) is the causative agent of Johne’s disease, an economically important disease in ruminants worldwide. It was first isolated in Egypt in 2005. Since then, the pathogen has been detected in different Egyptian provinces. In order to trace the source of infection, genotyping using simple methods of high discriminatory power such as mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTR) were carried out in different countries. Until now there is no published information about MIRU-VNTR genotyping of MAP isolates in Egypt. To address that point, 100 faecal samples were collected and cultivated from 3 different suspected dairy farms. Fourteen isolates belonging to one farm were identified as MAP and subjected to genotyping using 8 different MIRU-VNTR loci PCRs. Two different genotypes were recognized based on size polymorphism observed in one locus (VNTR-7) that was confirmed by sequencing. Our work provides a preliminary basis of constructing a MIRU-VNTR genotyping database of MAP in Egypt.

 
Keyword(s): EGYPT, INMV, MIRU-VNTR, PARATUBERCULOSIS
 
 
References: 
  • ندارد
  •  
 
Click to Cite.
APA: Copy

FAWZY, A., & FAYED, A., & YOUSSEF, H., & EL SAYED, A., & ZSCHOCK, M. (2016). SHORT PAPER: FIRST REPORT OF MIRU-VNTR GENOTYPING OF MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS ISOLATES FROM EGYPT. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 17(2 (55)), 130-133. https://www.sid.ir/en/journal/ViewPaper.aspx?id=507931



Vancouver: Copy

FAWZY A., FAYED A., YOUSSEF H., EL SAYED A., ZSCHOCK M.. SHORT PAPER: FIRST REPORT OF MIRU-VNTR GENOTYPING OF MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS ISOLATES FROM EGYPT. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR). 2016 [cited 2021May08];17(2 (55)):130-133. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507931



IEEE: Copy

FAWZY, A., FAYED, A., YOUSSEF, H., EL SAYED, A., ZSCHOCK, M., 2016. SHORT PAPER: FIRST REPORT OF MIRU-VNTR GENOTYPING OF MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS ISOLATES FROM EGYPT. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), [online] 17(2 (55)), pp.130-133. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507931.



 
  pdf-File
Yearly Visit 57
 
Latest on Blog
Enter SID Blog