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Paper Information

Journal:   IRANIAN JOURNAL OF ANIMAL SCIENCE RESEARCH   WINTER 2016 , Volume 7 , Number 4; Page(s) 504 To 511.
 
Paper: 

THE EFFECTS OF CONTINUOUS AND INTERMITTENT FEEDING OF β-AGONIST ZILPATEROL HYDROCHLORIDE ON MUSCLE B-ADRENERGIC RECEPTORS GENE EXPRESSION IN FEEDLOT MALE LAMBS

 
 
Author(s):  VAHEDI V.*, SADEGHI M., HEDAYAT EVRIGH N., NAJAFPANAH M.J., DIRANDEH E.
 
* DEPARTMENT OF ANIMAL SCIENCE, MOGHAN COLLEGE OF AGRICULTURE AND NATURAL RESOURCES, UNIVERSITY OF MOHAGHEGH ARDABILI, ARDABIL
 
Abstract: 

Introduction: The compounds known as b-adrenergic agonists (b-AA) are organic molecules that have the ability to bind to b-adrenergic receptors (b-AR) and start biochemical reactions that will result in increase of accretion of skeletal muscle and reduction in accretion of fat. The anabolic responses to b-AA are temporary, with a peak time occurring during the first 14 days, after which there is a linear decline in growth response, due to either down-regulation or desensitization of the b-AR. Based on down-regulation of b-AR research, the hypothesis that intermittent feeding of b-AA could enhance response on growth performance was created. The objective of this study was therefore to ascertain the effects of continuous and intermittent use of ZH for a period of 42 d on b-AR gene expression in Lori-Bakhtiari feedlot lambs.
Materials and Methods: The continuous feeding of ZH (daily regimen), intermittent 1 d feeding ZH followed by 1 d of withdrawal (1 on 1 off regimen), and intermittent 2 d feeding ZH followed by 2 d of withdrawal (2 on 2 off regimen) were employed as the different feeding methods. Thirty two Lori-Bakhtiari male lambs (initial BW=44
±4.7 kg) were assigned to one of four treatments (8 lambs/treatment) based on initial BW and were fed with a diet content of 14% protein and supplemented with 0.2 mg/kg of live weight d-1ZH. The basal diet without ZH was the added control group. For evaluating gene expression, biopsy samples of the semimembranosus muscle were collected from 3 lambs per treatment before ZH supplementation on d 0, and subsequently at d 21, and d 42. Samples were rapidly frozen in liquid N2. In laboratory after total RNA isolation from muscle, the RNA was then reverse-transcribed into complementary DNA (cDNA). Real time PCR for cDNA samples was performed using an iQ5 BioRad instrument.
Results and Discussion: The results of this study showed that the main effects and period × regimen interactions effect was not significant on
b1 and b2-adrenergic receptors gene expression. Also there were no significant differences between treatments and control group. Most of the physiological effects of catecholamines are due to specific interactions with b-adrenergic receptors (b-AR). The growth response to b-AA administration commonly is often reduced over time which is possibly a result of desensitization of the b-AR after chronic continuous exposure to the b-AA. The modification in the dosage of b-AA during the feeding period was previously proven to be a useful approach for compensating the reduced response in rats and lambs. Likewise, Hossner (2005) suggested that the intermittent treatment, e.g. treating for 2 days and resting for 2 days is one of the solutions for avoiding the desensitization and downregulation of receptors. In studies evaluating mRNA responses to b-AA, no change occurred in b1-AR mRNA abundance due to b-AA treatment. Baxa et al. (2010) explained that no detectable changes of b1-AR could be attributed to generally low abundance, which results in differences hidden by sample-to-sample variation. We were unable to detect differences in the mRNA expression of b2-AR mRNA in muscle samples. The decrease in b2-AR protein expression due to ZH treatment may be in response to a posttranscriptional event.
Conclusion: According to our results, it can be concluded that we could use ZH intermittently (1 on 1 off-42 d) in feedlot lambs to decrease fattening costs versus of daily administration of ZH. Further investigation is suggested to detect the alteration of
b-adrenergic receptors in continuous and intermittent feeding regimens for clarifying possibly down-regulation or desensitization of them.

 
Keyword(s): β-ADRENERGIC RECEPTORS, FEEDLOT LAMBS, RT-PCR, ZILPATEROL HYDROCHLORIDE
 
 
References: 
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Citations: 
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APA: Copy

VAHEDI, V., & SADEGHI, M., & HEDAYAT EVRIGH, N., & NAJAFPANAH, M., & DIRANDEH, E. (2016). THE EFFECTS OF CONTINUOUS AND INTERMITTENT FEEDING OF β-AGONIST ZILPATEROL HYDROCHLORIDE ON MUSCLE B-ADRENERGIC RECEPTORS GENE EXPRESSION IN FEEDLOT MALE LAMBS. IRANIAN JOURNAL OF ANIMAL SCIENCE RESEARCH, 7(4), 504-511. https://www.sid.ir/en/journal/ViewPaper.aspx?id=507770



Vancouver: Copy

VAHEDI V., SADEGHI M., HEDAYAT EVRIGH N., NAJAFPANAH M.J., DIRANDEH E.. THE EFFECTS OF CONTINUOUS AND INTERMITTENT FEEDING OF β-AGONIST ZILPATEROL HYDROCHLORIDE ON MUSCLE B-ADRENERGIC RECEPTORS GENE EXPRESSION IN FEEDLOT MALE LAMBS. IRANIAN JOURNAL OF ANIMAL SCIENCE RESEARCH. 2016 [cited 2021July29];7(4):504-511. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507770



IEEE: Copy

VAHEDI, V., SADEGHI, M., HEDAYAT EVRIGH, N., NAJAFPANAH, M., DIRANDEH, E., 2016. THE EFFECTS OF CONTINUOUS AND INTERMITTENT FEEDING OF β-AGONIST ZILPATEROL HYDROCHLORIDE ON MUSCLE B-ADRENERGIC RECEPTORS GENE EXPRESSION IN FEEDLOT MALE LAMBS. IRANIAN JOURNAL OF ANIMAL SCIENCE RESEARCH, [online] 7(4), pp.504-511. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507770.



 
 
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