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Paper Information

Journal:   PHARMACEUTICAL SCIENCES   2012 , Volume 18 , Number 1; Page(s) 69 To 78.
 
Paper: 

COMPARATIVE STUDY ON THE EFFECT OF OBTAINED CELL WALL AND CYTOPLASMIC FRACTION FROM SACCHAROMYCES CEREVISIAE AND SACCHAROMYCES BOULARDII ON K562 CELL LINE

 
 
Author(s):  BONIADI F., TUKMECHI AMIR*, NEJATI V.
 
* DEPARTMENT OF PATHOBIOLOGY AND QUALITY CONTROL, ARTEMIA AND AQUATIC ANIMALS RESEARCH INSTITUTE, URMIA UNIVERSITY, URMIA, IRAN
 
Abstract: 

Objectives: The goal of this study was to evaluate in vitro anti turmeric properties of extracted cell wall and cytoplasmic fraction from Saccharomyces cerevisiae and S. boulardii as probiotic and comparative their effect on chronic myeloid leukemia (K562) cell line.
Methods: S. cerevisiae and S. boulardii were cultured in a medium containing yeast extract and disrupted with sonicator and centrifuged for separation the cell wall from cytoplasmic fraction. Then different concentrations of cell wall and cytoplasmic fraction were prepared and their anti tumeric properties assayed with MTT technique by using Doxorubicin as positive control.
Results: Our result showed both cell wall and cytoplasmic fraction that obtained from S. cerevisiae and S. boulardii have anti turmeric effect on K562. Statistical analysis indicated that Yeast cell wall could inhibit the growth of K562 than cytoplasmic fraction at level of p<0.05. Also, we found that, prepared compounds from S. boulardii have high anti turmeric properties than S. cerevisiae, but there was not seen any differences (p<0.05).
Conclusion: Based on obtained results we conclude that the extraction of cell wall from both microorganisms has suitable application for inhibiting chronic myeloid leukemia, but these anti tumeric properties would warrant further study on the clinical application of yeast cell wall.

 
Keyword(s): S. CEREVISIAE, S. BOULARDII, CELL WALL, CYTOPLASMIC FRACTION, ANTI TURMERIC PROPERTIES, K562 CELL LINE
 
 
References: 
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Click to Cite.
APA: Copy

BONIADI, F., & TUKMECHI, A., & NEJATI, V. (2012). COMPARATIVE STUDY ON THE EFFECT OF OBTAINED CELL WALL AND CYTOPLASMIC FRACTION FROM SACCHAROMYCES CEREVISIAE AND SACCHAROMYCES BOULARDII ON K562 CELL LINE. PHARMACEUTICAL SCIENCES, 18(1), 69-78. https://www.sid.ir/en/journal/ViewPaper.aspx?id=507700



Vancouver: Copy

BONIADI F., TUKMECHI AMIR, NEJATI V.. COMPARATIVE STUDY ON THE EFFECT OF OBTAINED CELL WALL AND CYTOPLASMIC FRACTION FROM SACCHAROMYCES CEREVISIAE AND SACCHAROMYCES BOULARDII ON K562 CELL LINE. PHARMACEUTICAL SCIENCES. 2012 [cited 2021May15];18(1):69-78. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507700



IEEE: Copy

BONIADI, F., TUKMECHI, A., NEJATI, V., 2012. COMPARATIVE STUDY ON THE EFFECT OF OBTAINED CELL WALL AND CYTOPLASMIC FRACTION FROM SACCHAROMYCES CEREVISIAE AND SACCHAROMYCES BOULARDII ON K562 CELL LINE. PHARMACEUTICAL SCIENCES, [online] 18(1), pp.69-78. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507700.



 
 
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