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Paper Information

Journal:   PHARMACEUTICAL SCIENCES   2012 , Volume 18 , Number 1; Page(s) 51 To 60.
 
Paper: 

SCREENING AND ISOLATION OF CEPHALOSPORIN ACYLASE ENZYME-PRODUCING BACTERIA AND OPTIMIZATION OF ENZYME PRODUCTION PROCESS

 
 
Author(s):  FEIZI H., FARAJNIA SAFAR*, SHAPOURI R., HEMATI S., AZIMI H.
 
* BIOTECHNOLOGY RESEARCH CENTER, TABRIZ UNIVERSITY OF MEDICAL SCIENCES, TABRIZ, IRAN
 
Abstract: 

Objectives: Cephalosporin acylases are highly specialized peptidases, which are capable for cleaving the amide bond between a cephalosporin nucleus and its side chain. This enzyme is used in the enzymatic conversion of cephalosporin C to the cephem nucleus (7-ACA).7-ACA is a key intermediate for manufacturing of semi synthetic cephalosporins in the pharmaceutical industries. Thus, achieving an efficient, safe and economical production routes is of great economical and bio-environmental importance.
Methods: The aim of this study was to screen soil bacteria for identification of cephalosporin acylase producing pseudomonas strains with spectrophotometric method.
In spectrophotometric method, the enzyme activity, production of 7-ACA and formation of imine group with
rDAB are determined by a colorimetric reaction. After isolation and identification of enzyme producing strain, the effect of incubation time, temperature, culture medium pH and glutaric acid concentration on production of enzyme were studied.
Results: In this research, out of 215 soil-isolated strains, only one strain showed noticeable cephalosporin acylase activity. Selected strain showed optimized activity by incubation at 35°C for 48 hours in a pH=8 and in the presence of 2 gram/liter glutaric acid.
Conclusion: Findings of this study indicates that spectrophotometric method is an effective and rapid method for identification and isolation of cephalosporin acylase producing microorganisms.

 
Keyword(s): CEPHALOSPORIN ACYLASE, 7-AMINOCEPHALOSPORANIC ACID, OPTIMIZATION, PSEUDOMONAS
 
 
References: 
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Click to Cite.
APA: Copy

FEIZI, H., & FARAJNIA, S., & SHAPOURI, R., & HEMATI, S., & AZIMI, H. (2012). SCREENING AND ISOLATION OF CEPHALOSPORIN ACYLASE ENZYME-PRODUCING BACTERIA AND OPTIMIZATION OF ENZYME PRODUCTION PROCESS. PHARMACEUTICAL SCIENCES, 18(1), 51-60. https://www.sid.ir/en/journal/ViewPaper.aspx?id=507696



Vancouver: Copy

FEIZI H., FARAJNIA SAFAR, SHAPOURI R., HEMATI S., AZIMI H.. SCREENING AND ISOLATION OF CEPHALOSPORIN ACYLASE ENZYME-PRODUCING BACTERIA AND OPTIMIZATION OF ENZYME PRODUCTION PROCESS. PHARMACEUTICAL SCIENCES. 2012 [cited 2021May07];18(1):51-60. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507696



IEEE: Copy

FEIZI, H., FARAJNIA, S., SHAPOURI, R., HEMATI, S., AZIMI, H., 2012. SCREENING AND ISOLATION OF CEPHALOSPORIN ACYLASE ENZYME-PRODUCING BACTERIA AND OPTIMIZATION OF ENZYME PRODUCTION PROCESS. PHARMACEUTICAL SCIENCES, [online] 18(1), pp.51-60. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507696.



 
 
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