Paper Information

Journal:   JOURNAL OF FISHERIES SCIENCE AND TECHNOLOGY (JFST)   WINTER 2016 , Volume 4 , Number 4; Page(s) 51 To 63.
 
Paper: 

THE EFFECT OF SOME CHEMICAL FACTORS ON SERINOPROTEINASES ACTIVITY OF TRYPSIN AND CHYMOTRYPSIN IN CASPIAN BROWN TROUT (SALMO TRUTTA CASPIUS) FRY

 
 
Author(s):  ZAMANI ABBAS*
 
* FISHERIES DEPARTMENT, FACULTY OF NATURAL RESOURCES AND ENVIRONMENTAL, MALAYER UNIVERSITY, MALAYER, IRAN
 
Abstract: 

The effect of metal ions, surfactant, oxidizing agents and enzyme inhibitors was considered on trypsin and chymotrypsin activity of the Caspian brown trout fry. The results showed K+ and Na+ didn’t significanly decrease trypsin and chymotrypsin activity (p>0.05). Ca2+ and Mg2+ significantly increased trypsin and chymotrypsin activity (p<0.05). Mn2+,Cu2+,Ba2+,Co2+,Zn2+,Fe2+ and Al3+ significantly decreased the activity of trypsin and chymotrypsin (p<0.05). Saponin and taurocholic acid significantly increased trypsin and chymotrypsin activity. Sodium cholate significantly increased chymotrypsin activity (p<0.05), but not the trypsin activity (p>0.05). Oxidizing agents, including hydrogen peroxide and sodium perborate, significantly decreased trypsin (p<0.05). Trypsin and chymotrypsin activity significantly decreased in the presence of SBTI, PMSF and r-Aminobenzamidine inhibitors (p>0.05). The inhibitors such as TPCK, pepstatinA, iodoacetic acid, EDTA and b-mercaptoethanol did not significantly decrease the trypsin activity (p>0.05), but they significantly decreased chymotrypsin activity (p<0.05). Trypsin activity in the presence of TLCK showed a significant decrease (p<0.05), but TLCK and pepstatin A had no significant effect on chymotrypsin activity (p>0.05).

 
Keyword(s): FRY, CASPIAN BROWN TROUT, CHEMICAL FACTORS, CHYMOTRYPSIN
 
References: 
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