Paper Information

Journal:   JUNDISHAPUR JOURNAL OF NATURAL PHARMACEUTICAL PRODUCTS   MAY 2016 , Volume 11 , Number 2; Page(s) 0 To 0.
 
Paper: 

GROWTH INHIBITION AND APOPTOSIS INDUCTION BYVINCETOXICUM PUMILUM DECNE. ON HL-60 AND K562 LEUKEMIC CELL LINES

 
 
Author(s):  TAYARANI NAJARAN ZAHRA, MOUSAVI SEYED HADI, RASOULINEJAD MARZIEH, ASILI JAVAD, IRANSHAHY MILAD, EMAMI SEYED AHMAD*
 
* DEPARTMENT OF PHARMACOGNOSY, SCHOOL OF PHARMACY, MASHHAD UNIVERSITY OF MEDICAL SCIENCES, MASHHAD, I.R. IRAN
 
Abstract: 

Background: Phenanthroindolizidine alkaloids have been isolated from many species of Vincetoxicum. These alkaloids show promising anti-cancer activity and are a current topic of interest.
Objectives: The inhibition of cell viability by different extracts of V. pumilum was measured in vitro in HL-60 and K562 human leukemic cancer cell lines and in freshly-isolated peripheral blood lymphocytes as a normal cell line.
Materials and Methods: Using resazurin staining, cell viability was measured. In addition, the presence of apoptotic cells was determined using propidium iodide (PI) staining of DNA fragments (sub-G1 peak). Employing western blot analysis, levels of Bax, Bcl-2, and PARP cleavage were measured.
Results: TheCH2Cl2 fraction of V.pumilum showeda potent dose-related inhibitory effectoncell viability. TheCH2Cl2 fractionshowed IC50 values of 8.3 and 12.5
mg/mL in the K562 and HL-60 cell lines, respectively. A sub-G1 peak in the flow cytometry histograms of treated cells induced by the CH2Cl2 fraction suggested an induction of apoptosis. Further, both the parent methanol extract and its CH2Cl2 sub-fraction increased the expression of the pro-apoptotic protein Bax and of cleaved PARP in the cells.
Conclusions: Collectively, the V. pumilum extracts showed remarkable cytotoxic activity and merit further investigation as anticancer agents.

 
Keyword(s): APOCYNACEAE, CYTOTOXICITY, LEUKEMIC CELL LINES, APOPTOSIS, VINCETOXICUM PUMILUM
 
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