Paper Information

Journal:   PETROLEUM RESEARCH   WINTER 2016 , Volume 25 , Number 85-2; Page(s) 76 To 87.
 
Paper: 

STUDY OF DECOLORIZATION ABILITY OF ORANGE AZO β-NAPHTOL BY HALOMONAZ STRAIN GB BACTERIA AND OPTIMIZATION THE EFFECTS OF CONCENTRATION, PH AND TEMPERATURE

 
 
Author(s):  ENTEZARI GHAZALEH*, OTADI MARYAM, AMIRI RAHEBEH
 
* DEPARTMENT OF CHEMICAL ENGINEERING, ENGINEERING COLLEGE, SCIENCE AND RESEARCH UNIT OF ISLAMIC AZAD UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Through this research, the ability of orange Azo b-NapHtol’s decolorization by Halomonaz Strain Gb bacteria and the efect of conectration, PH adn temoeratureon decolorization studied. The optimal conditions for decolorization were obtained via changing the level of different factors while keeping one factor in each test constant, is assayed. The final result shows the maximum percentage of decolorization (88%) by 40 ppm concentration, 38oC and pH = 9 after 6 days of process. As a result of testing in a wide range of concentration (15-125 ppm), decolorization ability of the bacteria divided in two parts: examinations with concentration of less than 40 ppm and more than 40 ppm. The most effective factor was pH and increasing pH in a range of 6-10 caused a remarkable increase in decolorization. Temperature of 35-40oC did not too have much effect on the decolorization. Comparison of arising spectrums from HPLC and UV-Vis before and after decolorization and observing the totomery ph enomenon in arising spectrums from IR confirms the considerable decolorization. Because of halopHilic and halotolerant bacteria, the amount of %5 (W/V) Sodium Chloride was added during all the tests.

 
Keyword(s): HALOPHICLIC AND HALOTOERANT, HALOMONAZ STRAIN GB BACTERIA, DECOLORIZATION, AZO B-NAPHTOL ORANGE, AZO DYE
 
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