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Paper Information

Journal:   INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE   SPRING 2016 , Volume 5 , Number 2; Page(s) 80 To 89.
 
Paper: 

CARCINOEMBRYONIC ANTIGEN EXPRESSION AND RESISTANCE TO RADIATION AND 5-FLUOROURACIL-INDUCED APOPTOSIS AND AUTOPHAGY

 
 
Author(s):  EFTEKHAR EBRAHIM, JABERIE HAJAR, NAGHIBALHOSSAINI FAKHRADDIN*
 
* DEPARTMENT OF BIOCHEMISTRY, SHIRAZ UNIVERSITY OF MEDICAL SCIENCES, SCHOOL OF MEDICINE, SHIRAZ, IRAN
 
Abstract: 

Understanding the mechanism of tumor resistance is critical for cancer therapy. In this study, we investigated the effect of carcinoembryonic antigen (CEA) overexpression on UV-and 5-fluorouracil (5-FU) -induced apoptosis and autophagy in colorectal cancer cells. We used histone deacetylase (HDAC) inhibitor, NaB and DNA demethylating agent, 5-azacytidine (5-AZA) to induce CEA expression in HT29/219 and SW742 colorectal cancer cell lines. MTT assay was used to measure IC50 value of the cells exposed to graded concentrations of 5-FU with either 0.1 mM NaB or 1mM 5-AZA for 72 h. Using CHO- and SW742-CEA transfectants, we also investigated the effect of CEA expression on UV- and 5-FU-induced apoptosis and autophagy. Treatment of HT29/219 cell line with NaB and 5-AZA increased CEA expression by 29% and 31%, respectively. Compared with control cells, the IC50 value for 5-FU of NaB and 5-AZA-treated cells increased by 40% and 57%, respectively. Treatment of SW742 cells with NaB or 5-AZA increased neither CEA expression nor the IC50 value for 5-FU. In comparison to parental cells, CEA expression also significantly protected transfected cells against UV-induced apoptosis. Decreased proportions of autophagy and apoptosis were also observed in 5-FU treated SW742- and CHO-CEA transfectants. We conclude that CEA expression can effectively protect colorectal cancer cells against radiation and drug-induced apoptosis and autophagy.

 
Keyword(s): CARCINOEMBRYONIC ANTIGEN (CEA), COLORECTAL CANCER, 5-FLUOROURACIL, APOPTOSIS, AUTOPHAGY
 
 
References: 
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Click to Cite.
APA: Copy

EFTEKHAR, E., & JABERIE, H., & NAGHIBALHOSSAINI, F. (2016). CARCINOEMBRYONIC ANTIGEN EXPRESSION AND RESISTANCE TO RADIATION AND 5-FLUOROURACIL-INDUCED APOPTOSIS AND AUTOPHAGY. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, 5(2), 80-89. https://www.sid.ir/en/journal/ViewPaper.aspx?id=507443



Vancouver: Copy

EFTEKHAR EBRAHIM, JABERIE HAJAR, NAGHIBALHOSSAINI FAKHRADDIN. CARCINOEMBRYONIC ANTIGEN EXPRESSION AND RESISTANCE TO RADIATION AND 5-FLUOROURACIL-INDUCED APOPTOSIS AND AUTOPHAGY. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE. 2016 [cited 2021May07];5(2):80-89. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507443



IEEE: Copy

EFTEKHAR, E., JABERIE, H., NAGHIBALHOSSAINI, F., 2016. CARCINOEMBRYONIC ANTIGEN EXPRESSION AND RESISTANCE TO RADIATION AND 5-FLUOROURACIL-INDUCED APOPTOSIS AND AUTOPHAGY. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, [online] 5(2), pp.80-89. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507443.



 
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