Paper Information

Journal:   JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)   MAY 2016 , Volume 9 , Number 5; Page(s) 0 To 0.
 
Paper: 

ASSESSMENT OF THE HUMAN CYTOMEGALOVIRUS UL97 GENE FOR IDENTIFICATION OF RESISTANCE TO GANCICLOVIR IN IRANIAN IMMUNOSUPPRESSED PATIENTS

 
 
Author(s):  KEYVANI HOSSEIN, TAGHINEZHAD SAROUKALAEI SEDIGHEH, MOHSENI AMIR HOSSEIN*
 
* RESEARCH AND DEVELOPMENT DEPARTMENT, KEYVAN VIROLOGY SPECIALTY LABORATORY (KVSL), TEHRAN, IR IRAN
 
Abstract: 

Background: Human cytomegalovirus (HCMV) infections are a major cause of morbidity and mortality among immunocom promised patients. Prolonged antiviral therapy is a cause of mutation and drug resistance in the HCMV genome.
Objectives: The aim of this study was to identify resistance to ganciclovir (GCV) in Iranian immunosuppressed patients at two different stages of the disease: early (before GCV is initiated) and late (after six months of GCV therapy).
Patients and Methods: In this study, 87 specimens from Iranian patients were amplified using nested PCR amplification of the UL97 gene. Sequence analyses of products were performed for identifying the mutated codons.
Results: The present study show that the most frequent GCV-resistant mutations occurred in codons A594V (26.43%), H520Q (18.39%), and M460V (13.79%), consequently occurring at a low frequency in the L595S (2.29%), E596G (1.14%), and Del 594 (1.14%) codons, and with intermediate frequency in the C592G (10.34%), M460I (9.19%), and C603W (6.89%) codons. We describe for the first time a new GCV-resistance mutation, the deletion of codon 594, in the UL97 gene of Iranian HCMV patients after GCV therapy, following renal transplantation.
Conclusions: The findings of the present study can be utilized to detect GCV resistance patterns among Iranian immunocompromised patients and to treat HCMV infections accordingly.

 
Keyword(s): UL97 PROTEIN, HUMAN CYTOMEGALOVIRUS, GANCICLOVIR, IRAN
 
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