Paper Information

Journal:   JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)   MAY 2016 , Volume 9 , Number 5; Page(s) 0 To 0.
 
Paper: 

THE DOMINANCE OF PILUS ISLET 1 IN PNEUMOCOCCAL ISOLATES COLLECTED FROM PATIENTS AND HEALTHY INDIVIDUALS

 
 
Author(s):  KHODAEI FARZANEH, AHMADI ALI, SAYAHFAR SHIRIN, IRAJIAN GHOLAMREZA, TALEBI MALIHEH*
 
* DEPARTMENT OF MICROBIOLOGY, SCHOOL OF MEDICINE, IRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IR IRAN
 
Abstract: 

Background: Pili in Streptococcus pneumoniae have been shown to be one of the adherence factors for epithelial cells in the human upper respiratory tract. Two types of pilus-like structures (pilus islet-1 and pilus islet-2) have been distinguished in S. pneumoniae.
Objectives: To investigate the presence of pilus islet-1 (PI-1) in S. pneumoniae and the correlation between our isolates.
Materials and Methods: In this study, 162 S. pneumoniae isolates were collected from clinical specimens, and normal flora were also examined for the distribution of PI-1 using the presence of the rlrA and rrgC genes as markers for this islet and sipA as an indicator of pilus islet-2 (PI-2). BOX-PCR analyses were performed to determine the genetic relationship between isolates.
Results: The results confirmed the presence of rlrA and rrgC genes in both clinical (n = 39) and normal flora (n = 26) isolates. The minimal inhibitory concentration results revealed that the rate of resistance of these isolates to the three antibiotics tested ranged from 26% for penicillin to 46% for erythromycin and tetracycline. Furthermore, 12% of the isolates were resistant to all three antibiotics. Strain typing using repetitive element BOX-PCR analysis among the 65 isolates identified 8 different band patterns.
Conclusions: Our results indicated that the dissemination of PI-1 was widespread in S. pneumoniae isolates, although no PI-2 isolates were detected. Furthermore, the frequency of rlrA and rrgC of clinical isolates was significantly more than that of normal flora isolates.

 
Keyword(s): PILUS, BOX-PCR, STREPTOCOCCUS PNEUMONIA
 
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