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Paper Information

Journal:   JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)   MAY 2016 , Volume 9 , Number 5; Page(s) 0 To 0.
 
Paper: 

BETA-LACTAMASE ENCODED GENES BLATEM AND BLACTX AMONG ACINETOBACTER BAUMANNII SPECIES ISOLATED FROM MEDICAL DEVICES OF INTENSIVE CARE UNITS IN TEHRAN HOSPITALS

 
 
Author(s):  KHALILZADEGAN SARA, SADE MOJTABA*, GOUDARZI HOSSEIN, ESLAMI GITA, HALLAJZADEH MASOUMEH, FALLAH FATEMEH, YADEGARYNIA DAVOOD
 
* INTERNATIONAL BRANCH, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IR IRAN
 
Abstract: 

Background: Excessive consumption of antimicrobial materials in hospitals is considered as the main encoder leading to the emergence, development and acquisition of new bacterial resistance to beta-lactamase.
Objectives: Owing to the lack of proper information regarding the mechanism of the bacterial resistance to antibiotics and responsible genes in the country, the current study aimed to consider the resistance or sensitivity of the Acinetobacter baumannii multi drug resistant (MDR) isolates facing 2% glutaraldehyde. The study was conducted in the selected intensive care units in Tehran hospitals, Iran, in 2013.
Materials and Methods: In this study conducted over a period of 10 months, A. baumannii species were isolated by bacterial culture following biochemical tests from intensive care units (ICUs) of some hospitals in Tehran, Iran (Fayazbaksh, Taleghani, ImamKhomeini, Valiasr, Labafinejad). The resistance and sensitivity of the isolates to antibiotics were considered according to the clinical and laboratory standard institute CLSI (2012) guidelines. By multiplex PCR method, blaCTX and blaTEM genes were detected and finally, MDR strains were treated with 2% glutaraldehyde. PCR was used for each strain of MDR using specific primers.
Results: In the current study, 131 A. baumannii isolates (22.3%) out of 588 were studied. The level of resistance to various antibiotics was in the range of 69.4% to 100%. The frequencies of blaTEM and blaCTX genes were 3.2% and 19.4%, respectively. MIC50% and MIC90% of imipenem and meropenem antibiotics were 32
±1 mg/mL and 64±1 mg/mL, respectively (P < 0.9). However no resistance to glutaraldehyde was observed. Different bands of MDR strains were observed in the PCR product by electrophoresis.
Conclusions: It seems that besides the variety and prevalence of blaTEM and blaCTX, enormous mechanisms such as porin and leaking systems (efflux pumps) are responsible for the information of the A. baumannii resistance to disinfectants. The study on an accurate consideration of the resistance in strains and other microorganisms is advised.

 
Keyword(s): LACTAMASE GENES, ANTIMICROBIAL RESISTANCE, DISINFECTANTS, ACINETOBACTER BAUMANNII
 
 
References: 
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Click to Cite.
APA: Copy

KHALILZADEGAN, S., & SADE, M., & GOUDARZI, H., & ESLAMI, G., & HALLAJZADEH, M., & FALLAH, F., & YADEGARYNIA, D. (2016). BETA-LACTAMASE ENCODED GENES BLATEM AND BLACTX AMONG ACINETOBACTER BAUMANNII SPECIES ISOLATED FROM MEDICAL DEVICES OF INTENSIVE CARE UNITS IN TEHRAN HOSPITALS. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 9(5), 0-0. https://www.sid.ir/en/journal/ViewPaper.aspx?id=507393



Vancouver: Copy

KHALILZADEGAN SARA, SADE MOJTABA, GOUDARZI HOSSEIN, ESLAMI GITA, HALLAJZADEH MASOUMEH, FALLAH FATEMEH, YADEGARYNIA DAVOOD. BETA-LACTAMASE ENCODED GENES BLATEM AND BLACTX AMONG ACINETOBACTER BAUMANNII SPECIES ISOLATED FROM MEDICAL DEVICES OF INTENSIVE CARE UNITS IN TEHRAN HOSPITALS. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM). 2016 [cited 2021May06];9(5):0-0. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507393



IEEE: Copy

KHALILZADEGAN, S., SADE, M., GOUDARZI, H., ESLAMI, G., HALLAJZADEH, M., FALLAH, F., YADEGARYNIA, D., 2016. BETA-LACTAMASE ENCODED GENES BLATEM AND BLACTX AMONG ACINETOBACTER BAUMANNII SPECIES ISOLATED FROM MEDICAL DEVICES OF INTENSIVE CARE UNITS IN TEHRAN HOSPITALS. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), [online] 9(5), pp.0-0. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507393.



 
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