Paper Information

Journal:   JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY   SPRING 2016 , Volume 2 , Number 1; Page(s) 1 To 14.
 
Paper: 

ASPERGILLUS NIGER MEASUREMENT IN TOMATO PASTE USING REAL TIME

 
 
Author(s):  KARAZHYAN REZA*, HABIBI NAJAFI MOHAMMAD BAGHER, YAVARMANESH MASOUD, EDALATIAN MOHAMMAD REZA, POURIANFAR HAMIDREZA
 
* FOOD QUALITY AND SAFETY RESEARCH DEPARTMENT, FOOD SCIENCE AND TECHNOLOGY RESEARCH INSTITUTE
 
Abstract: 

In this study, molecular method used to measure the Aspergillus niger with Real Time PCR technique in tomato paste. Because of problems mold count method such as proofer error, low and non-repeatability precision, accuracy and repeatability, developing a standard method high accuracy and repeatability and mold counts seems necessary. Real Time PCR molecular method can be used as a suitable method to identify and quantify the precise mold is considered based on the measurement of absolute and relative genes existing molds. Tomato paste samples with different amounts of mold spores (101, 103 and 105) per gram were contaminated. After incubation and growth of spores and mycelium of mold, DNA extraction from each sample was performed using al-sammari method. Then, using Real Time PCR reaction of DNA copy number of molds using SYBR Green reagent and primers of the Aspergillus niger genus was determined. The results showed thatby increasing amount of DNA molds in controls and inoculated samples Real Time curve were entered logarithmic phase in the lower CT values. The correlation coefficient of linear calibration Real Time was R2=0.938 which indicates that the accuracy of this test is quantitative detection. The reaction specificity was determined using melting curve of the primers and indicated that the reaction has been completely dedicated.

 
Keyword(s): ASPERGILLUS NIGER, QUANTIFICATION, REAL TIME PCR
 
References: 
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