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Paper Information

Journal:   REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY   APRIL 2016 , Volume 4 , Number 2; Page(s) 89 To 97.
 
Paper: 

CONSTRUCTION OF A NOVEL DNA VACCINE CANDIDATE ENCODING AN HSPX-PPE44-ESXV FUSION ANTIGEN OF MYCOBACTERIUM TUBERCULOSIS

 
 
Author(s):  MORADI BAGHER, SANKIAN MOJTABA, AMINI YOUSEF, MESHKAT ZAHRA*
 
* IMMUNOLOGY RESEARCH CENTER, MASHHAD UNIVERSITY OF MEDICAL SCIENCES, MASHHAD, IRAN
 
Abstract: 

Background: Mycobacterium tuberculosisis the causative agent of tuberculosis (TB). Bacille Calmette-Guerin (BCG) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult TB vaccine. The aim of this study was to develop a new tuberculosis DNA vaccine candidate encoding a recombinant HspX-PPE44-EsxV fusion antigen of M. tuberculosis.
Methods: A fusion DNA segment consisting of HspX, linker, PPE44, linker, and EsxV, after codon optimization, was designed. The fusion DNA was cloned and its sequence confirmed. Then, expression of a recombinant pcDNA3.1 (+) /HspX-PPE44-EsxV plasmid in Chinese hamster ovary (CHO) cells was verified by RT-PCR and Western-blot analysis.
Results: A 1968 bp band in RT-PCR and a 68 kDa band on Western-blot analysis confirmed transcription and expression of recombinant hspX - ppe44 - esxV in eukaryotic cells.
Conclusions: A recombinant DNA segment encoding the HspX-PPE44-EsxV fusion antigen of M. tuberculosis was constructed and considered to be tested as a new TB DNA vaccine candidate.

 
Keyword(s): DNA VACCINE, ESX, HSP, MYCOBACTERIUM TUBERCULOSIS, PPE44
 
 
References: 
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+ Click to Cite.
APA: Copy

MORADI, B., & SANKIAN, M., & AMINI, Y., & MESHKAT, Z. (2016). CONSTRUCTION OF A NOVEL DNA VACCINE CANDIDATE ENCODING AN HSPX-PPE44-ESXV FUSION ANTIGEN OF MYCOBACTERIUM TUBERCULOSIS. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 4(2), 89-97. https://www.sid.ir/en/journal/ViewPaper.aspx?id=507356



Vancouver: Copy

MORADI BAGHER, SANKIAN MOJTABA, AMINI YOUSEF, MESHKAT ZAHRA. CONSTRUCTION OF A NOVEL DNA VACCINE CANDIDATE ENCODING AN HSPX-PPE44-ESXV FUSION ANTIGEN OF MYCOBACTERIUM TUBERCULOSIS. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY. 2016 [cited 2021July25];4(2):89-97. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507356



IEEE: Copy

MORADI, B., SANKIAN, M., AMINI, Y., MESHKAT, Z., 2016. CONSTRUCTION OF A NOVEL DNA VACCINE CANDIDATE ENCODING AN HSPX-PPE44-ESXV FUSION ANTIGEN OF MYCOBACTERIUM TUBERCULOSIS. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, [online] 4(2), pp.89-97. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=507356.



 
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