Paper Information

Journal:   BASIC AND CLINICAL NEUROSCIENCE   JULY 2016 , Volume 7 , Number 3; Page(s) 249 To 257.
 
Paper: 

5-HT2A SEROTONIN RECEPTOR DENSITY IN ADULT MALE RATS’ HIPPOCAMPUS AFTER MORPHINE-BASED CONDITIONED PLACE PREFERENCE

 
 
Author(s):  MOHAMMADI RABIEH, JAHANSHAHI MEHRDAD*, JAMEIE SEYED BEHNAMEDIN
 
* DEPARTMENT OF ANATOMY, SCHOOL OF ANATOMY, NEUROSCIENCE RESEARCH CENTER, FACULTY OF MEDICINE, GOLESTAN UNIVERSITY OF MEDICAL SCIENCES, GORGAN, IRAN
 
Abstract: 

Introduction: A close interaction exists between the brain opioid and serotonin (5-HT) neurotransmitter systems. Brain neurotransmitter 5-HT plays an important role in the regulation of reward-related processing. However, a few studies have investigated the potential role of 5-HT2A receptors in this behavior. Therefore, the aim of the present study was to assess the influence of morphine and Conditioned Place Preference (CPP) on the density of 5-HT2A receptor in neurons of rat hippocampal formation.
Methods: Morphine (10 mg/kg, IP) was injected in male Wistar rats for 7 consecutive days (intervention group), but control rats received just normal saline (1 mL/kg, IP). We used a hotplate test of analgesia to assess induction of tolerance to analgesic effects of morphine on days 1 and 8 of injections. Later, two groups of rats were sacrificed one day after 7 days of injections, their whole brains removed, and the striatum and PFC immediately dissected. Then, the NR1 gene expression was examined with a semi-quantitative RT-PCR method.
Results: Our data showed that the maximum response was obtained with 2.5 mg/kg of morphine. The density of 5-HT2A receptor in different areas of the hippocampus increased significantly at sham-morphine and CPP groups (P<0.05). On the other hand, the CPP groups had more 5-HT2A receptors than sham-morphine groups and also the sham-morphine groups had more 5-HT2A receptors than the control groups.
Conclusion: We concluded that the phenomenon of conditioned place preference induced by morphine can cause a significant increase in the number of serotonin 5-HT2A receptors in neurons of all areas of hippocampus.

 
Keyword(s): CONDITIONED PLACE PREFERENCE, MORPHINE, 5-HT2A RECEPTORS, HIPPOCAMPUS, RATS
 
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