Paper Information

Journal:   BASIC AND CLINICAL NEUROSCIENCE   JULY 2016 , Volume 7 , Number 3; Page(s) 241 To 247.
 
Paper: 

MORPHINE-INDUCED ANALGESIC TOLERANCE EFFECT ON GENE EXPRESSION OF THE NMDA RECEPTOR SUBUNIT 1 IN RAT STRIATUM AND PREFRONTAL CORTEX

 
DOI: 

10.15412/J.BCN.03070309

 
Author(s):  AHMADI SHAMSEDDIN*, RAFIEENIA FATEMEH, ROSTAMZADEH JALAL
 
* DEPARTMENT OF BIOLOGICAL SCIENCE AND BIOTECHNOLOGY, FACULTY OF SCIENCE, UNIVERSITY OF KURDISTAN, SANANDAJ, IRAN
 
Abstract: 

Introduction: Morphine is a potent analgesic but its continual use results in analgesic tolerance. Mechanisms of this tolerance remain to be clarified. However, changes in the functions of m-opioid and N-Methyl-D-aspartate (NMDA) receptors have been proposed in morphine tolerance. We examined changes in gene expression of the NMDA receptor subunit 1 (NR1) at mRNA levels in rat striatum and prefrontal cortex (PFC) after induction of morphine tolerance.
Methods: Morphine (10 mg/kg, IP) was injected in male Wistar rats for 7 consecutive days (intervention group), but control rats received just normal saline (1 mL/kg, IP). We used a hotplate test of analgesia to assess induction of tolerance to analgesic effects of morphine on days 1 and 8 of injections. Later, two groups of rats were sacrificed one day after 7 days of injections, their whole brains removed, and the striatum and PFC immediately dissected. Then, the NR1 gene expression was examined with a semi-quantitative RT-PCR method.
Results: The results showed that long-term morphine a administration induces tolerance to analgesic effect of the opioid, as revealed by a significant decrease in morphine-induced analgesia on day 8 compared to day 1 of the injections (P<0.001). The results also showed that the NR1 gene expression at mRNA level in rats tolerant to morphine was significantly increased in the striatum (P<0.01) but decreased in the PFC (P<0.001).
Conclusion: Therefore, changes in the NR1 gene expression in rat striatum and PFC have a region-specific association with morphine-induced analgesic tolerance.

 
Keyword(s): MORPHINE, GENE EXPRESSION, PREFRONTAL CORTEX, CORPUS STRIATUM
 
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