Paper Information

Journal:   JENTASHAPIR JOURNAL OF HEALTH RESEARCH (JENTASHAPIR)   FEBRUARY 2016 , Volume 7 , Number 1; Page(s) 1 To 9.
 
Paper: 

ALGORITHM-BASED FETAL GENDER DETERMINATION USING X AND Y MINI-SHORT TANDEM REPEATS AT EARLY GESTATIONAL AGES

 
DOI: 

10.17795/jjhr.29365

 
Author(s):  AGHANOORI MOHAMAD REZA, MOHAMMADZADEH SHAHRIARY GHAZALEH, KHORRAMI MEHDI, SAYADI MEHRAB, YAZDANI YASAMAN, YAZDANI SAMAN, AKHOND MOHAMAD REZA, GOODARZI HAMEDREZA*
 
* DEPARTMENT OF MEDICAL GENETICS, SHIRAZ UNIVERSITY OF MEDICAL SCIENCES, SHIRAZ, IR IRAN
 
Abstract: 

Background: Detection of fetal DNA in maternal blood has been examined by many research groups for a few years; thereby, scientists have a shorter way to take to approach prenatal diagnosis of abnormal pregnancies. The Y chromosome sequences have recently become the most common applicable indices for fetal sex determination.
Objectives: We conducted an algorithmic X and Y mini-Short Tandem Repeats (STRs) genotyping method that could solve the problem of false negative (no detection of Y sequences) results of previous methods.
Patients and Methods: Blood samples were obtained from 106 pregnant women and their spouses. Conventional PCR amplified 19 mini-Short Tandem Repeats (STRs) and three non-STR markers, which were subsequently genotyped by the means of Polyacrylamide gel electrophoresis (PAGE).
Results: Sensitivity and specificity of the mini-STR genotyping method for fetal DNA detection were calculated (95.9% and 98%, respectively) with a confidence interval of 95%. In addition, sensitivity and informative ness were computed for each of the single mini-STR markers in our conventional PCR method. We also introduced the minimum number of mini-STRs needed to reach maximum validity for fetal gender determination in clinical settings.
Conclusions: Algorithm-based mini-STR genotyping method significantly increases the reliability (sensitivity and specificity) of gender determination using free fetal DNA and could be applied in prenatal clinical testing.

 
Keyword(s): FREE FETAL DNA, SEX DETERMINATION, X-Y ALGORITHM, MINI-STR
 
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