Paper Information

Journal:   TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)   FEBRUARY 2016 , Volume 73 , Number 11; Page(s) 819 To 826.
 
Paper: 

CANDIDA COLONIZATION AND SPECIES IDENTIFICATION BY TWO METHODS IN NICU NEWBORN

 
 
Author(s):  TAHERZADEH NARGES SADAT, ZAINI FARIDEH, DAIE GHAZVINI ROSHANAK, REZAIE SASAN, MAHMOUDI MAHMOUD, KADIVAR MALIHEH, NAYERI FATEMEH SADAT, SAFARA MAHIN, KORDBACHEH PARIVASH*
 
* POURSINA ST., GHODS ST., ENGHELAB AVE., SCHOOL OF PUBLIC HEALTH, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background: Over the last two decades invasive candidiasis has become an increasing problem in neonatal intensive care units (NICUs). Colonization of skin and mucous membranes withCandida spp. is important factor in the pathogenesis of neonatal infection and several colonized sites are major risk factors evoking higher frequencies of progression to invasive candidiasis. The aim of this study was to detectCandida colonization in NICU patients.
Methods: This cross-sectional study was conducted on 93 neonates in NICUs at Imam Khomeini and Children Medical Center Hospitals in Tehran. Cutaneous and mucous membrane samples obtained at first, third, and seventh days of patients’ stay in NICUs during nine months from August 2013 to May 2014. The samples were primarily cultured on CHROMagar Candida medium. The cultured media were incubated at 35°C for 48h and evaluated based on colony color produced on CHROMagar Candida. In addition, isolated colonies were cultured on Corn Meal Agar medium supplemented with tween 80 for identification ofCandida spp. based on their morphology. Finally, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was performed for definite identification of isolated species.
Results: Colonization by Candida spp. was occurred in 20.43% of neonates. Fifteen and four patients colonized with one and two differentCandida spp., respectively. Isolated Candida spp.
identified as; C. parapsilosis (n: 10), C. albicans (n: 7), C. tropicalis (n: 3), C. guilliermondii (n: 2), and C. krusei (n: 1). In present study non-albicans Candia species were dominant (69.56%) andC. parapsilosis was the most frequent isolate (43.47%). Using Fisher's exact test, the correlation between fungal colonization with low birth weight, low gestational age, and duration of hospital stay was found to be statistically significant (P=0.003).
Conclusion: The results of this study imply to the candida species colonization of neonates.
Neonates in NICU are at the highest risk for severe infection withCandida parapsilosis.
Therefore, isolation of C. parapsilosis as the most common species (43.47%) in present study was noteworthy.

 
Keyword(s): CANDIDA, CANDIDA PARAPSILOSIS, COLONIZATION, NEONATAL INTENSIVE CARE UNITS, POLYMERASE CHAIN REACTION
 
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