Paper Information

Journal:   TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)   FEBRUARY 2016 , Volume 73 , Number 11; Page(s) 778 To 783.
 
Paper: 

THE PREVALENCE OF METHICILLIN-RESISTANT STAPH. AUREUS STRAINS PRODUCING ENTEROTOXIN A AND B

 
 
Author(s):  ABBASI SAFIYEH*, TAEI SASSAN, ZAMANZAD BEHNAM
 
* DEPARTMENT OF MICROBIOLOGY, IMMUNOLOGY, STUDENT'S RESEARCH COMMITTEE, SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, SHAHREKORD, IRAN
 
Abstract: 

Background: Staphylococcus aureus is a gram positive coccus which is able to cause different kinds of infection in certain condition. The function of this bacteria is to provide the conditions for the invasion of it to the host with the secretion of different sorts of toxins such asStaphylococcus aureus enterotoxin, including important virulence factors that super antigens are all factors digestive inconvenience. Staphylococcus aureus enterotoxin-secreting toxins such conditions provides invasion of host genes. There are different types of SE, but type A enterotoxin (SEA) and type B enterotoxin (SEB) are the most important types. Therefore, in this study, the prevalence ofStaphylococcus aureus toxin-producing enterotoxin genes (SEB, SEA) in clinical strains isolated from patients in teaching hospitals of Shahrekord city, Iran, were studied.
Methods: This cross-sectional and descriptive study, which was conducted from May 2014 to December 2014. A hundred and ten isolates ofStaphylococcus aureus from patients collected over a period of 8 months and were first identified using standard biochemical methods and laboratory. Using standard methods and laboratory tests were identified and compared with the antibiotic oxacillin minimum inhibitory concentration were determined by broth micro dilution, and then they were assessed by polymerase chain reaction (PCR) technique.
Results: The results indicated that, 110 samples of dairy products infected by Staphylococcus aureuswere detected. Two cases (1.8%) of these infected samples were carrying both enterotoxin A and enterotoxin B genes. The frequencies of enterotoxin A genes were twenty-six cases (23.6%) and The frequencies of enterotoxin B genes were two cases (1.8%), respectively.
Conclusion: The detection of enterotoxin A and enterotoxin B genes, shows the most important role they have in bringing about superinfection. The detection of enterotoxin A and B genes, shows the most important role they have in bringing about superinfection.
Enterotoxins SEA and SEB are heat stable; therefore heating has no effect on dairy products contaminated by enterotoxins and gastritis may occur in a short period of time. As PCR is a rapid, sensitive, specific and inexpensive method, we suggest that it can be replaced to traditionally assays for detectingStaphylococcus aureus enterotoxin.

 
Keyword(s): METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS, STAPHYLOCOCCAL ENTEROTOXIN A, STAPHYLOCOCCAL ENTEROTOXIN B.
 
References: 
  • ندارد
 
  Yearly Visit 66
 
Latest on Blog
Enter SID Blog