Paper Information

Journal:   TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)   FEBRUARY 2016 , Volume 73 , Number 11; Page(s) 767 To 777.
 
Paper: 

EXAMINING THE EFFECTS OF PLATELET-RICH PLASMA AND PLATELET-RICH FIBRIN ON AUTOLOGOUS FULL THICKNESS SKIN GRAFT SURVIVAL IN RATS

 
 
Author(s):  LATIFI NOORAHMAD, REZVANI NAVID, FATEMI MOHAMMAD JAVAD*, NOURIAN MAJID, ARAGHI SHIRIN, BAGHERI TORAN
 
* BURN RESEARCH CENTER, SHAHID MOTAHARI HOSPITAL, YASEMI ST., VALI´ASR AVE., TEHRAN, IRAN
 
Abstract: 

Background: Graft survival has been considered the major problem in reconstructive surgery. Clinical studies have helped us to understand the role of PRP in increasing skin survival. Our goal in this study was to examine the treatment effects of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) on autologous full thickness skin graft survival in male rats.
Methods: This experimental study was performed on 36 rats of Sprague-Dawley race with weighing approximately 250 to 300 gr on May 2015 in animal laboratory of Hazrat Fatima Hospital. After anesthesia, rats were divided into 3 groups. We injected platelet-rich plasma (PRP) in the first group, platelet-rich fibrin (PRF) in the second and saline in the third group after removing the skin. Microscopic analysis was performed with camera (Canon powershot SX200, Tokyo, Japan) on days 7, 14, 21 and 28 after surgery. We used image analysis system (ImageJ, ver.1.45) to examine necrosis and survival rate. Samples were studied with H& E staining on day 28 microscopically for histological analysis of vascular density and angiogenesis.
Results: Our findings showed the area of necrosis in animals injected with PRP on days 7 and 14, was meaningfully less than control group (P=0.0001). There was no meaningful difference between control and PRP groups (P>0.05). The area of necrosis in animals injected with PRF did not have any significant difference with control group from beginning to 21st day (P<0.0001). there was no meaningful difference in vascular density between control and PRP group, whereas in animals injected with PRF the vascular density was significantly less than control group (P=0.002).
Conclusion: According to our results in this study, we can conclude that using autologous PRP can enhance the process of healing soft tissue injury and be affective at increasing graft survival. This method is suggested to be conducted for patients highly at risk of graft loss and also for those who are in need of early treatments.

 
Keyword(s): PLATELET-RICH FIBRIN, PLATELET-RICH PLASMA, RATS, SKIN GRAFT, SURVIVAL
 
References: 
  • ندارد
 
  Yearly Visit 58
 
Latest on Blog
Enter SID Blog