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Paper Information

Journal:   TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)   MAY 2016 , Volume 74 , Number 2; Page(s) 92 To 98.
 
Paper: 

THE EXPRESSION LOSS OF GPIBα DUE TO ECTODOMAIN SHEDDING IN PRP DERIVED PLATELET CONCENTRATES DURING STORAGE

 
 
Author(s):  PIRMOHAMMAD JAMAAT ZEYNAB, HOSSEINI EHTERAMOLSADAT, GHASEMZADEH MEHRAN*
 
* BLOOD TRANSFUSION RESEARCH CENTER, HIGH INSTITUTE FOR RESEARCH AND EDUCATION IN TRANSFUSION MEDICINE, IRANIAN BLOOD TRANSFUSION ORGANIZATION BUILDING, HEMMAT EXPRESS WAY, NEXT TO THE MILAD TOWER, TEHRAN, IRAN
 
Abstract: 

Background: Platelet adhesion typically occurs by the critical role of GPIb-V-IX in capturing free-flowing platelets to the injured vessel wall where its rapid binding kinetics enables platelet tethering even under conditions of high shear through the interaction of the major ligand-binding subunit of GPIb-V-IX, GPIb? with subendothelial-bound vWF. During storage, platelet undesired activation may lead to platelet storage lesion (PSL) which changes the expression levels of platelet functional receptors including GPIb?. This study investigates the levels of expression and ectodomain shedding of platelet adhesive receptor GPIba during the storage of platelet rich plasma (PRP) concentrates (PRP- PCs).
Methods: Five PRP-platelet concentrates were obtained from Iranian Blood Transfusion Organization (IBTO). The GPIb
a expressions of platelets were analyzed on day 1, 3 and 5 after storage using flowcytometry. To examine the ectodomain shedding of this receptor the microparticle free supernatants obtained from stored platelets were subjected to western blot analysis. For control study, blood specimens was drawn from healthy consenting individuals and resting platelets were isolated while resuspended in Tyrode buffer.
Results: Our results indicated a continuous decrease of GPIb
a expression during storage where the expression from fist day (Mean fluorescence intensity=86±5.9) was significantly reduced compared to that of fifth day (mean fluorescence intensity=61±7.7) after storage (P=0.0094). Conversely, shed GPIba (Glycocalicin) demonstrated continuous elevation during five-day storage (P=0.0098). According to the results the shedding levels for the first day were increased from 0.31±0.3 to 1.5±0.4 by the day 5 after storage.
Conclusion: Our study has demonstrated significant loss of platelet GPIb
a during storage mostly due to receptor ectodomain shedding that leads to significant increase of soluble GPIba in stored platelets. Considering the high levels of shed GPIba in long stored platelets whether the transfusion of such products might be associated with defective adhesive function of platelets or possible proinflammatory effects could be of interests for future investigation.

 
Keyword(s): GLYCOCALICIN, GPIBα, PLATELET ACTIVATION, PLATELET-RICH PLASMA, THROMBOSIS
 
 
References: 
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Click to Cite.
APA: Copy

PIRMOHAMMAD JAMAAT, Z., & HOSSEINI, E., & GHASEMZADEH, M. (2016). THE EXPRESSION LOSS OF GPIBα DUE TO ECTODOMAIN SHEDDING IN PRP DERIVED PLATELET CONCENTRATES DURING STORAGE. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 74(2), 92-98. https://www.sid.ir/en/journal/ViewPaper.aspx?id=506977



Vancouver: Copy

PIRMOHAMMAD JAMAAT ZEYNAB, HOSSEINI EHTERAMOLSADAT, GHASEMZADEH MEHRAN. THE EXPRESSION LOSS OF GPIBα DUE TO ECTODOMAIN SHEDDING IN PRP DERIVED PLATELET CONCENTRATES DURING STORAGE. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ). 2016 [cited 2021May13];74(2):92-98. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=506977



IEEE: Copy

PIRMOHAMMAD JAMAAT, Z., HOSSEINI, E., GHASEMZADEH, M., 2016. THE EXPRESSION LOSS OF GPIBα DUE TO ECTODOMAIN SHEDDING IN PRP DERIVED PLATELET CONCENTRATES DURING STORAGE. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), [online] 74(2), pp.92-98. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=506977.



 
 
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