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Paper Information

Journal:   ARCHIVES OF IRANIAN MEDICINE   January 2003 , Volume 6 , Number 1; Page(s) 23 To 28.
 
Paper: 

POLYMERASE CHAIN REACTION-SEQUENCE SPECIFIC PRIMER (PCR-SSP) VERSUS SEROLOGY IN TYPING OF HLA-A,B AND -C IN IRANIAN PATIENTS

 
 
Author(s):  NAROUEINEZHAD M.*, KHOSRAVI F., DANESH A., NIKBIN B.
 
* TEHRAN-IRAN
 
Abstract: 
Background-Histocompatibillity locus antigen (HLA) class i typing by serology is the ‎most common method in the routine clinical setting .With DNA sequence information ‎available for alleles of the HLA system and development of molecular biological ‎techniques , it is possible to tissue type for allelic different in HLA genes.
‎ Methods- in this study, serological HLA-A, -B and -C typing results were compared ‎to typing results obtained using sequence -specific primers in the polymerase chain ‎reaction (PCR-SSP).HLA-A, -B and -C typing was performed on a random Iranian ‎population consisting of 40 healthy individuals.
‎ Results- There were 16 blank antigens ( antigen not detected ) on serological testing , ‎‎9 in the A locus and 7 in the B locus .PCR-SSP allowed assignment of 2 of the 9 ‎blanks in the A locus and 3 of the 7 blanks in the B locus. In this study, there was a ‎‎31% difference in the two typing methods.
‎ Conclusion- DNA typing is necessary, particularly in individuals with uncommon ‎alleles and immunosuppression.‎ ‎
 
Keyword(s): HLA TYPING.LYMPHOCYTOTOXICITY TEST.PCR
 
References: 
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