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Paper Information

Journal:   ARCHIVES OF IRANIAN MEDICINE   January 2003 , Volume 6 , Number 1; Page(s) 29 To 31.
 
Paper: 

EVALUATION OF RAPID "DIPSTICK RK39" TEST IN DIAGNOSIS AND SEROLOGICAL SURVEY OF VISCERAL LEISHMANIASIS IN HUMANS AND DOGS IN IRAN

 
 
Author(s):  EDRISIAN GH.H.*, SHAMSSI S., MOHEBALI M., HAJARAN H., MAMISHI S., DESJEUX P.
 
* 
 
Abstract: 
‎. Background and Objective - Visceral leishmaniasis is endemic in northwest and ‎southern Iran and is found sporadically in other parts of the country. It manifests as ‎the Mediterranean type of kala-azar, which mainly affects children. Early diagnosis ‎and prompt treatment of the disease could prevent its high mortality. We evaluated the ‎rapid "Dipstick rK39" test in diagnosis and serological survey of the disease in human ‎as well as in dogs, and compared it either with the direct agglutination test (DAT) or ‎enzyme-linked immunosorbent assay (ELISA).‎ Methods - "Dipstick rK39" kits were used for diagnosis in suspected and confirmed ‎kala-azar patients and for case finding among children in the endemic area of ‎Meshkin-Shahr, Ardebil Province, in northwest Iran. The kits were also used to ‎diagnose visceral leishmaniasis in dogs experimentally infected with Leishmania ‎infantum as well as in dogs with clinical symptoms of infection in endemic areas. ‎Results were evaluated based either on results with DAT, ELISA or, in a few cases, ‎microscopic diagnosis.‎ Results - The rapid "Dipstick rK39" test was sufficiently sensitive and quite specific ‎in diagnosis of visceral leishmaniasis in human as well as in dog.‎ Conclusion - The "Dipstick rK39" test is rapid and noninvasive. It does not require ‎much expertise or elaborate equipment and it can be used for diagnosis of visceral ‎leishmaniasis in remote endemic areas.‎
 
Keyword(s): DIPSTICK RK39E DOG .HUMAN A IRAN .VISCERAL LEISHMANIASIS
 
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