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Paper Information

Journal:   ARCHIVES OF RAZI INSTITUTE   December 2003 , Volume - , Number 56; Page(s) 1 To 10.
 
Paper: 

RESRICTION ENZYME ANALYSIS OF TYMIDINE KINASE GENE IN FOUR IRANIAN ISOLATES OF HERPES SIMPLEX VIRUS TYPE 1

 
 
Author(s):  PISHRAFT SABET L., ROUSTAEI M.H.*, ZANDI K., BAMDAD T.
 
* Virology Dept, Faculaty of Medical Science Tarbiat Modarres University, Tehran, Iran
 
Abstract: 

The thymidine kinase (TK) gene of four Iranian herpes simplex virus-I isolate was amplified and the electrophoretic patterns of the gene were compared after digestion with six restriction endonucleases including ApaI, Bg/I, DraII, EcoRV and PstI. DN8A of virus was first extracted separately by lysis buffer and phenol/chloroform method. Their TK genes were amplified using a pair of special primers, which were designed by Gene 8Runner software. The polymerase chain reaction product of each TK gene was digested with the restriction enzymes separately and electrophoresed in polyacrylamide gel. The results indicate that there was no difference among the electrophoretic patterns of the isolates TK gene.

 
Keyword(s): HERPES SIMPLEX VIRUS-1, RFLP, THYMIDINE KINASE
 
 
References: 
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Citations: 
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APA: Copy

PISHRAFT SABET, L., & ZANDI, K., & ROUSTAEI, M., & BAMDAD, T. (2003). RESRICTION ENZYME ANALYSIS OF TYMIDINE KINASE GENE IN FOUR IRANIAN ISOLATES OF HERPES SIMPLEX VIRUS TYPE 1. ARCHIVES OF RAZI INSTITUTE, -(56), 1-10. https://www.sid.ir/en/journal/ViewPaper.aspx?id=4466



Vancouver: Copy

PISHRAFT SABET L., ZANDI K., ROUSTAEI M.H., BAMDAD T.. RESRICTION ENZYME ANALYSIS OF TYMIDINE KINASE GENE IN FOUR IRANIAN ISOLATES OF HERPES SIMPLEX VIRUS TYPE 1. ARCHIVES OF RAZI INSTITUTE. 2003 [cited 2021November30];-(56):1-10. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=4466



IEEE: Copy

PISHRAFT SABET, L., ZANDI, K., ROUSTAEI, M., BAMDAD, T., 2003. RESRICTION ENZYME ANALYSIS OF TYMIDINE KINASE GENE IN FOUR IRANIAN ISOLATES OF HERPES SIMPLEX VIRUS TYPE 1. ARCHIVES OF RAZI INSTITUTE, [online] -(56), pp.1-10. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=4466.



 
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