Paper Information

Journal:   TEB VA TAZKIEH   Winter 2004 , Volume - , Number 51; Page(s) 10 To 16.
 
Paper: 

IDENTIFICAION OF A FACTOR INHIBITING PROSTATE CANCER CELL LINE (LNCAP)

 
 
Author(s):  ABOU ALHASANI MOHSEN*
 
* PASTEUR INSTITUTE OF IRAN
 
Abstract: 

A new antiproliferative activity from the conditional medium of two androgen-independent prostatic cancer cell lines, PC3 and DU-145, was identified. This antiproliferative activity selectively inhibited cell proliferation of an androgen-dependent prostate cancer cell line LNCaP in a dose dependent manner. No antiproliferative activity was observed against mouse fibro blast 3T3, normal human lymphocytes, human leukemic cells including promyelocytes HL-6 and T-cells HUT-78, or human adenocarcinoma cell lines, including prostatic cells JCA-1 ovary NIH: OVCAR-3, cervix C-33A and breast MDA-MB-231. Cell cycle analysis revealed that the antiproliferative activity did not induce apoptosis in LNCaP cells, but it prevented some of G1 LNCaP cells from entering into the S-Phase of the cell cycle. The antiproliferative activity was sensitive to protease digestion and its approximate molecular weight was between 50-100 kDa by Amicon filters. The antiproliferative activity was not affected by neutralizing antibodies against TGF- b1,2,3 TNF-a, PDGF, EGF, IL-1 IL-2. IL-3, IL-4, or IL-6.

 
Keyword(s): PROSTATE CANCER, ANTI GROWTH FACTOR, CANCER CELLS DU-145, PC3, LNCAP
 
References: 
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