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Paper Information

Journal:   AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)   Spring 2003 , Volume 10 , Number 1; Page(s) 25 To 29.
 
Paper: 

COMPARISON THE SENSITIVITY OF ELISA AND DIG-ELISA FOR SERODIAGNOSIS OF HUMAN TOXOPLASMOSIS

 
 
Author(s):  DALIMI A.*, SOLHJOO K., GHAFFARIFAR FATEMEH
 
* Department of Parasitology, School of Medical Sciences, Tarbiat Modarres University, Tehran , Iran
 
Abstract: 

Toxoplasmosis is one of the most common parasitic infection in human and other warm-blooded animals. Serological methods are reliable techniques actually used for diagnosis of toxoplasmosis. Comparison of sensitivity of ELISA and DIG-ELISA for diagnosis of human toxoplasmosis was the main objective of the present work. 68 human serum samples (48 positive sera and 20 negative sera) were tested using ELISA and DIG-ELISA (diffusion in Gel-ELISA). Among three dilutions of sera ‎‎(1:1, 1:10 and 1:100) tested for evaluation, the serum dilution 1:100 was selected as cut off. In this dilution, diameter of reaction zone was 6.08/ mm. In ELISA, 1:200 serum dilution was selected as cut off and at this dilution OD was 0.735. The sensitivity and specificity of DIG-ELISA for serodiagnosis of toxoplasmosis at 1:100 serum dilution were 93.37%, 100%, respectively while the sensitivity and specificity of ELISA (at 1:200 serum dilution) were 91.66%, 85% respectively. The results of present study indicated that, DIG-ELISA in 1:100 serum dilutions, in comparison with ELISA proved higher sensitivity and specificity for diagnosis of toxoplasmosis‏.‏

 
Keyword(s):  DIG-ELISA, ELISA, HUMAN TOXOPLASMOSIS, SERODIAGNOSIS
 
References: 
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