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Paper Information

Journal:   JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)   2014 , Volume 27 , Number 1; Page(s) 144 To 154.
 
Paper: 

EFFECT OF APPLE PECTIN OR PECTIC ACID AND MODIFIED CITRUS PECTIN ON NO RELEASE AND INHIBITION OF CELL PROLIFERATION IN RAT PITUITARY TUMOR CELLS GH3/B6

 
 
Author(s):  MOGHTADERI H., SEPEHRI H., REZAYOF A., DELPHI L., DASHTBOZORGI S.
 
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Abstract: 

Nitric oxide (NO) can both induce and suppress apoptosis according to the physiological conditions. Recent investigations have indicated that pectin derivatives, which are polysaccharides in the cell wall of plants, are able to induce apoptosis in cancerous cells. In the present study the amount of NO release from rat pituitary tumor cell line GH3/B6 after treatment with deferent concentration of modified citrus pectin (MCP), pectic acid (PA), SNP (as positive control) and L-NAME (as negative control) is under investigation. The cells were cultured in Ham’s F12 medium which was completed with 10% FBS and treated with different concentration of MCP and AP. The cell proliferation inhibition rate was determined with MTT assay. The amount of NO release was assessed by an indirect method of using Griess reagent. Our results demonstrated that incubation of GH3/B6 cells with 1mg/ml PA for 4 hours increased the amount of NO release up to 40% more than control otherwise none of MCP concentrations affect the secretion of NO. In addition, MTT assay determined significant proliferation inhibition rate for the cells with 2.5, 5 mg/ml of PA and 3, 5 mg/ml of MCP after 24 and 48 hours incubation. So these results suggest that PA may inhibit cell proliferation via induction of NO release in GH3/B6 cells but MCP shows its inhibitory effect on cell proliferation by another pathway.

 
Keyword(s): PECTIC ACID, MODIFIED CITRUS PECTIN, NITRIC OXIDE, GH3/B6 CELL LINE
 
References: 
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