MYB gene family as one of the largest transcription factors in plants has a wide range of roles. One the most important functions of these genes is to regulate the anthocyanin level in different tissues. In apple (Malus´domestica), three genes MdMYB1, MdMYBA and MdMYB10 are responsible for developing red color by controlling anthocyanin level. In this study a variant of MdMYB10, named MdMYB10b, was isolated from an Iranian domestic red flesh apple and its expression pattern in various tissues was described. This gene was expressed in all red tissues of the plant including leaves, seed, fruit skin and flesh while no expression was detected in the control cultivar. These results coupled with bioinformatics examination of the genes which regulate anthocyanin in apple suggested that three MYBs (10, 1, A) are alleles of one locus. Furthermore, a specific expression pattern was presented based on this model: Alleles MdMYB1and MdMYBA are located in both red and white flesh genotypes and function only in the skin while MdMYB10 is located merely in red flesh genotype and function in all tissues.