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Paper Information

Journal:   JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)   2014 , Volume 27 , Number 1; Page(s) 88 To 98.
 
Paper: 

STUDY OF IN VITRO MICROSPORE EMBRYOGENESIS OF RAPESEED (BRASSICA NAPUS L. CV. PF704) WITH ELECTRON MICROSCOPY

 
 
Author(s):  ABDOLLAHI M.R.*
 
* AGRONOMY AND PLANT BREEDING DEPT., FACULTY OF AGRICULTURE, BU-ALI SINA UNIVERSITY, HAMEDAN I.R. OF IRAN
 
Abstract: 

In this research, ultrastructure of rapeseed (Brassica napus L.cv. PF704) microspores following the gametophytic and embryogenic developmental pathways in vitro was compared with the gametophytic development in vivo via electron microscopy. In reprogramming of rapeseed microspores to the embryogenesis pathway, there are some defined changes influencing the cell activities and structure. Some of these changes can be considered as microspore embryogenesis pathway in rapeseed. In this study, rapeseed microspores were isolated at different days after in vitro culture and prepared for electron microscopy studies. During the different developmental stages of rapeseed microspores, Differences in specific cellular properties such as cell size and shape, nuclear architecture, starch accumulation, presence of vacuoles were studied to characterize different stages of microspore embryogenesis and other pathways occurring in vitro. Results, showed the presence of abundant starch grains in a defined cytoplasmic region appeared as a specific property of the gametophytic development in vitro, as well as of the non-induced microspores of in vitro cultures under embryogenic-inductive conditions.

 
Keyword(s): BRASSICA NAPUS L. RAPESEED, MICROSPORE CULTURE, EMBRYOGENESIS, GAMETOPHYTIC DEVELOPMENT
 
References: 
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