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Paper Information

Journal:   JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)   2014 , Volume 27 , Number 1; Page(s) 26 To 34.
 
Paper: 

DETERMINATION CELLULASE ACTIVITY OF MUSHROOM COMPOST THERMOPHILIC ACTINOMYCETES BY FILTER PAPER ASSAY

 
 
Author(s):  TAGHIZADEH N.*, FARSI M., PAKDIN PARIZI A., TARIGHI S.
 
* BIOTECHNOLOGY DEPT., COLLAGE OF AGRICULTURE, FERDOWSI UNIVERSITY OF MASHHAD, MASHHAD, I.R. OF IRAN
 
Abstract: 

One of the most important substrate for producing of the edible white button mushrooms is compost. Thermophilic actinomycetes are one of the most important microflora in composting. So these actinomycetes were isolated from different steps in composting process by dilution technique. The isolates were incubated at 40oC. The chalky colonies were selected and transformed to new solid media to prepare pure culture of each isolate. Bacterial isolates were identified based on biochemical tests and amplified 16SrRNA restriction analysis technique. Ability of the isolates to break down cellulose, was assessed by using Filter paper in different temperature and pH. Maximum enzyme activity for each isolate were measured based on milligrams of glucose released from one milliliters extract. The isolates with high cellulase activity were screened for determination of the optimum pH and temperature. Isolate 23 had 1/10 mg/ml enzyme activity and it demonstrated a higher cellulase activity at 45oC. These isolates was applied for improvement quality compost.

 
Keyword(s): CELLULASE ACTIVITY, COMPOST, FILTER PAPER ASSAY, THERMOPHILIC ACTINOMYCETES
 
References: 
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