Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY)   2013 , Volume 26 , Number 2; Page(s) 184 To 195.
 
Paper: 

THE EFFECT OF LIGHT AND PLANT GROWTH REGULATORS ON CALLOGENESIS AND ANTHOCYANIN ACCUMULATION IN CALLI OF DIFFERENT EXPLANTS IN ROSA GALLICA

 
 
Author(s):  REZANEJAD F., TARRAHI R.
 
* 
 
Abstract: 

Callogenesis and anthocyanin content of vegetative explants such as leaf, stem, petiole and floral ones (petal, pistil, anther) from Rosa gallica were investigated under physical (light and dark) and chemical (different combinations of 2, 4-D, BAP and GA3) factors at different periods. The explants were cultured on the modified MS medium supplemented with vitamins and growth regulators. Cultures were exposed to dark and a 16.8 h (light dark) photoperiod at 1000 and 2000 lux light intensities. The calli (with the original explant) were then routinely subcultured onto fresh medium of the same composition at two weeks intervals. Combinations of 1mg l-1 BAP and 2- 3 mg l-1 2, 4-D were the most suitable treatment for callus induction in various explants. GA3 was resulted in callogenesis reduction. Callogenesis was started after 4 days in stem explants and after 8 days in the other explants. Calli grew and became massive and enlarged in the next weeks. Dark and 1000 and 2000 lux light intensities showed no significant different on callus induction although 2000 lux light intensity somewhat reduced callogenesis in anther and vegetative explants. Calli produced in light were red like and accumulated anthocynin. Anthocynin content was the higher in calli obtained from explants exposed to 2000 lux light intensity. Calli grown in dark were white to cremish, soft, fraible and contained a bit of anthocyanin.

 
Keyword(s): ANTHOCYANIN, CALLI, FLORAL EXPLANTS, LIGHT, ROSE
 
References: 
  • ندارد
 
  Persian Abstract Yearly Visit 67
 
Latest on Blog
Enter SID Blog