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Paper Information

Journal:   JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY)   2013 , Volume 26 , Number 2; Page(s) 176 To 183.
 
Paper: 

OPTIMIZATION OF STRAWBERRY POLLEN CULTURE AND ITS MAINTENANCE OF POLLEN GERMINATION CAPACITY

 
 
Author(s):  PIRI S., IMANI A., MAASOUMI H., BADRZADEH D.
 
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Abstract: 

Temperate fruits including small fruits, especially Strawberries are important fruit crops which mostly for fruit set needs the pollination of flowers and followed by pistil fertilization. Therefore, to achieve this act, pollen viability and its germination capability are essential. Examination of pollen viability and understand its quality is important. To optimize the pollen germination medium of Strawberry, an experimental was carried out with 9 types of culture medium containing different concentrations of boric acid (0, 25 and 50 mgL-1), sucrose (7.5, 10 and 15 %) and agar (2 %) in the In-vitro using random complete design with three replications. The results showed that maximum germination in combination medium B25S15 (25 mg boric acid and 15 % sucrose) with 87.66% germination was lowest germination medium in combination B0S7.5 (0 mg boric acid and 7.5 % sucrose) with 5.33% germination occurred. The viability of pollen Strawberry two months after maintenance at temperatures -20oC and -80oC showed that pollen stored at temperatures above maintain viability of pollen germination or no significant difference. Also, the results from pollen germination in selected culture medium showed that maximum pollen germination (83.93%) in laboratory condition was obtained and lowest pollen germination percentage in 10oC with 51.66 % occurred.

 
Keyword(s): IN-VITRO, POLLEN GERMINATION, STRAWBERRY
 
References: 
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