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Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   SUMMER 2012 , Volume 7 , Number 2 (29); Page(s) 193 To 201.
 
Paper: 

STUDY ON GENETIC DIVERSITY OF XANTHOMONAS ORYZAE PV. ORYZAE ISOLATES IN RICE FIELDS OF GILAN PROVINCE USING RAPD MARKER

 
 
Author(s):  TAHERI SAHRESTANI A.*, ELAHINIA S.A., NIKNEJAD KAZEMPOUR M., EBADI A.A.
 
* GILAN UNIVERSITY, DEPARTMENT OF AGRICULTURE, PLANT PROTECTION GROUP
 
Abstract: 

bacterial Leaf Blight of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), is an important disease throughout Asia. Yield losses of 10-70% have been recorded in severely infected fields.
In this study genetic variation in 60 isolates of Xoo, investigated using random amplified polymorphic DNA (RAPD) marker. As a whole all used markers amplified 187 polymorphic bands.
Analysis of molecular variance of RAPD data showed that the genetic variation within Xoo populations was greater than between populations. The similarity matrix made by Simple Matching coefficient and cluster analysis conducted by unweighted pair group method (UPGMA) with NTSYS-pc soft ware. At a similary index of 0.6 the isolates were grouped into 3 clusters. These results revealed that the RAPD markers which used in this study could differentiate nursery and field isolates from each other.

 
Keyword(s): BACTERIAL BLIGHT, GENETIC DIVERSITY, RAPD, RICE, ISOLATE, XANTHOMONAS ORYZAE PV. ORYZAE
 
References: 
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