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Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   SUMMER 2012 , Volume 7 , Number 2 (29); Page(s) 165 To 173.
 
Paper: 

ASSOCIATION ANALYSIS BETWEEN MORPHOMETRIC AND RAPD MARKERS IN SIAH MAHI, CAPOETA CAPOETA GRACILIS, WITHIN TAJAN RIVER

 
 
Author(s):  ANVARIFAR H., FARAHMAND H.*, NEMATOLLAHI M.A., RAHMANI H., KARAMI M., KHALILI B.
 
* DEPARTMENT OF FISHERIES, FACULTY OF NATURAL RESOURCES, UNIVERSITY OF TEHRAN, KARAJ, IRAN
 
Abstract: 

to investigate the relationships between morphometric and molecular markers as well as introduce morphometric characters that shown most genetic variations in Siah Mahi, Capoeta capoeta gracilis, have been designed. A total 65 fish specimens were collected by electeroshocker set that 35 individual related to up stream station and 31 individual related to down stream station. From ten random 10-mer primers, 6 primers which shown polymorphic pattern repeatable, were selected and use in the final analysis of two locations.26 morphometric factors were measured and was standardized. Totally 89 polymorphic band (marker) gained. Length of PCR products were between 50-1000 bp. In this study for first time in field aquaculture we used from Association analysis approach that in this method have been used from stepwise regression to determine relationship between each of morphometric characters and 89 genetic markers. In finally this results shown that 79 markers from 89 (86.51% of markers) shown significant relationship (P<0.05) to 18 morphometric characters that among them 9 characters shown significant relationship in high significant level (P?0.01) that we could use from this morphometric characters in topics related to population of fishes.

 
Keyword(s): ASSOCIATION ANALYSIS, MORPHOMETRIC TRAIT, RAPD MARKERS, SIAH MAHI, TAJAN RIVER
 
References: 
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