Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   SUMMER 2012 , Volume 7 , Number 2 (29); Page(s) 157 To 164.
 
Paper: 

NUCLEAR LOCALIZATION OF AGROBACTERIUM VIRD2 IN THE YEAST SACCHAROMYCES CEREVISIAE

 
 
Author(s):  SOLTANI J.*, LAL JONATHAN A., VAN HEUSDEN G. PAUL H., HOOYKAAS PAUL J.J.
 
* SECTION OF MOLECULAR AND DEVELOPMENTAL GENETICS, INSTITUTE OF BIOLOGY, LEIDEN UNIVERSITY, LEIDEN, THE NETHERLANDS
 
Abstract: 

upon infection of eukaryotic cells, Agrobacterium tumefaciens transfers a piece of its tumor inducing plasmid, T-DNA, to the host cell. The VirD2 virulence protein which is covalently bound to the T-DNA facilitates its transfer, nuclear localization and possibly integration into the host genome in collaboration with the interacting proteins of the host cell. VirD2 is essential for Agrobacterium-mediated transformation of both plants and non-plant cells. Here, using yeast Green Flourescent Protein (yGFP) technology we studied the subcellular localization of VirD2 expressed in yeast Saccharomyces cerevisiae cells. Fluorescence microscopy showed that an N-terminal yGFP fusion of VirD2, is located in the nucleus of yeast. This highlights nuclear localization of VirD2 in yeast and the importance of NLS sequences of C-terminal of VirD2 in this phenomenon.

 
Keyword(s): AGROBACTERIUM, GFP, SACCHAROMYCES CEREVISIAE, VIRD2
 
References: 
  • ندارد
 
  Persian Abstract Yearly Visit 73
 
Latest on Blog
Enter SID Blog