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Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   SUMMER 2012 , Volume 7 , Number 2 (29); Page(s) 129 To 140.
 
Paper: 

A STUDY OF GENE EXPRESSION AND FUNCTIONAL GENOMICS OF WHEAT, RICE, COTTON AND FESTUCA PLANTS UNDER DROUGHT STRESS BY ANALYZING EXPRESSED SEQUENCE TAGS (EST)

 
 
Author(s):  HEIDARY P.*, MALEKI ZANJANI B., HEIDARY SH.
 
* COLLEGE OF AGRICULTURE, ZANJAN UNIVERSITY, IRAN
 
Abstract: 

Drought stress is one of the most important preventions in the crop production. The goal of this study was to find the new effective genes in drought stress mechanism. For this EST libraries of wheat, rice, cotton and festuca plants under drought stress were analyzed. The EST data of four libraries were collected from harvard university databank. To find similarity between libraries, all of EST sequences were assembled by EGassembler software. Then all contigs were analyzed by blast x from CLC Protein Workbench software against non redundant protein presented in gene banks with Evalue?1*10-5. IDEG6 software was used for detecting genes with deferent expression pattern between libraries. The genes of lipid treansferase, phosphatase, metallothionein, dehydrin, glutathione s-transferase and LEA are important genes involved in drought stress in four libraries of wheat, rice, cotton and festuca.
Four libraries showed significant differences in functional catalogues of photosynthesis, oxidative pentose phosphate, mitochondrial electron transport, cell wall, amino acid metabolism, hormone metabolism, redox regulation, misc, RNA, CHO metabolism, stress, nucleotide metabolism, secondary metabolism, protein, signaling that explaining these four catalogues response to the drought stress with using different sub functional catalogues.

 
Keyword(s): DROUGHT STRESS, EST, FUNCTIONAL CATALOGUES, FUNCTIONAL GENOMICS, GENE EXPRESSION
 
References: 
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