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Paper Information

Journal:   ARCHIVES OF IRANIAN MEDICINE   NOVEMBER 2013 , Volume 16 , Number 11; Page(s) 636 To 641.
 
Paper: 

ASSOCIATION OF THE 223A/G LEPR POLYMORPHISM WITH SERUM LEPTIN LEVELS IN IRANIAN SUBJECTS WITH TYPE 2 DIABETES

 
DOI: 

0131611/AIM.005

 
Author(s):  MOHAMMADZADEH GHORBAN*, NIKZAMIR ABDOLRAHIM, MOHAMMADI JAVAD, POURDASHTI SARA, SHABAZIAN HAJEH, LATIFI SEYED MAHMOUD
 
* HYPERLIPIDEMIA RESEARCH CENTER, DEPARTMENT OF CLINICAL BIOCHEMISTRY, FACULTY OF MEDICINE, AHVAZ JUNDISHAPUR UNIVERSITY OF MEDICAL SCIENCES, AHVAZ, IRAN
 
Abstract: 

BACKGROUND: Leptin, an adipocyte-derived hormone, has a pivotal role in the regulation of body weight through acting on its specific leptin receptor (LEPR). The 223A/G polymorphism of the LEPR gene is one of the most common polymorphism in all populations.  In this study, we aimed to investigate the impact of the 223A/G polymorphism of the LEPR gene on serum levels of leptin in type 2 diabetes mellitus (T2DM) in a sample of Iranian population.
MATERIALS AND METHODS: One hundred and forty-four T2DM patients were screened and compared to 147 healthy controls. The 223A/G LEPR polymorphism was genotyped using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP). The serum levels of leptin were measured.
RESULTS: The mean serum levels of leptin in T2DM patients were significantly higher than that of healthy control subjects; 22.90 ng/ml (95% confidence interval [CI]=20.79 – 25.23) vs. 8.70 ng/ml (95% CI=7.87 – 9.63). The genotypes (AA, AG, and GG) distributions of the 223A/G polymorphism were 55.5%, 41%, and 3.5% in T2DM and 54.4%, 42.2%, and 3.4% in healthy controls. The results showed no significant differences in the 223A/G LEPR genotype and allele frequencies between T2DM and control subjects (X2=0.043, P=0.979 and X2=0.003, P=0.957), respectively. In addition, the serum leptin levels were markedly higher in subjects with GG genotype than those with AG or GG genotype only in T2DM
CONCLUSION: The 223A/G LEPR gene polymorphism is associated with markedly increased serum leptin levels in T2DM. However, no differences were determined in genotype and allele frequencies between T2DM patients and control subjects.

 
Keyword(s): LEPR, PCR-RFLP, T2DM
 
References: 
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