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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 78 To 78.
 
Paper: 

FREQUENCY OF MUTATIONS ASSOCIATED WITH RIFAMPICIN RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED FROM PATIENTS FROM WEST OF IRAN

 
 
Author(s):  MOHAJERI PARVIZ, SADRI HADIS*, NOROZI BAHARAK
 
* SCHOOL OF MEDICINE, KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES, KERMANSHAH, IRAN
 
Abstract: 

Background: Tuberculosis (TB) is a devastating infectious disease causing high mortality and morbidity worldwide. The most serious threat related to tuberculosis control is the recent emergence of drug-resistant tuberculosis strains. The aim of the present study was to identify various types of mutations in rpo B region from rifampicin resistant strains isolated from sputum of tuberculosis patients.
Methods: Drug susceptibility testing of 125 Mycobacterium tuberculosis isolates was determined using the CDC standard conventional proportional method. Target DNA of M. tuberculosis was amplified by PCR, hybridized and scanned. We used the LCD array to detect mutations within the 90 bp rpoB region. Each array is a transparent, pre-structured polymer supports using a non-fluorescent detection principle based on the precipitation of a clearly visible dark substrate.
Results: Of the 125 M. tuberculosis isolates, 35 (28%) were found to be rifampicin resistant and using the LCD array revealed point mutations at 9 different codons as follows S512T (AGC
®ACC) (20%) D516V (GAC®GTC) (20%) H526D (CAC®GAC) (6%) H526R (CAC®CGC) (20%) H526Y (CAC®TAC) (23%) S531W (TCG®TGG) (8%). The most frequent site mutations were L511P (CCG®CTG) (46%) followed S531l (TCG®TTG) (40%) and D516Y (GAC®TAC) (26%).
Conclusion: Phenotypic testing is time-consuming and needs laboratory facilities. Therefore, there is a need for rapid molecular methods for detection of mutation in drug resistance. Microarray rpoB can be used to detect rifampicin resistance determining region (RRDR) associated site mutations of rifampicin resistant M. tuberculosis isolates.

 
Keyword(s): MYCOBACTERIUM TUBERCULOSIS, RIFAMPICIN RESISTANCE, RPOB GENE
 
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