Background: Most Campylobacter infections are self-limiting but antimicrobial treatment (macrolides, fluoroquinolones) is necessary in severe cases. Rapidly evolving next-generation sequencing technologies may offer beneficial instrument in the future for the prediction of resistance in Campylobacter.
Methods: Pulsed-field gel electrophoresis (PFGE) has been widely used for molecular typing of C. jejuni because of its high discriminatory power. The PCR assay could identify Campylobacter -contaminated samples that were negative using the cultural method, agar dilution and broth micro dilution, while a disk diffusion method was recently standardized by the European Committee on Antimicrobial Susceptibility Testing.
Results: Pulsed-field gel electrophoresis (PFGE) method showed that there were several points of cross-contamination of product along the chain, and a high diversity of PFGE types with antimicrobial resistance to ciprofloxacin and tetracycline in the retail products. in PCR method we find susceptibility test results showed that 98.4% of isolates were resistant to one or more antimicrobial agents.in agar dilution and broth micro dilution method High levels of resistance to tetracycline and ciprofloxacin in humans, retail meats, and food animals are reported, but resistance to erythromycin and gentamicin in C. jejuni, which causes the vast majority of Campylobacter infections.
Conclusion: However, great strides have been made in the past decade in standardizing in vitro susceptibility testing methods for Campylobacter, the variety of methods and commentary criteria used reflects the need for further harmonization Campylobacter.