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Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   SPRING 2013 , Volume 8 , Number 1 (32); Page(s) 111 To 116.
 
Paper: 

STUDY ON POLYMORPHISM OF MYOSTATIN IN IRANIAN KARAKUL SHEEP BREED BY PCR-RFLP MARKER

 
 
Author(s):  ZARE J., MIRHOSSEINI S.Z.*
 
* GUILAN UNIVERSITY, IRAN
 
Abstract: 

The myostatin protein is a member of the transforming growth factor- b (TGF- b) that plays an important role in the development of the skeletall muscles. The myostatin gene is located on ovine chromosome 2 (OAR2) and consists of three exons and two introns. In some breeds, mutation of the myostatin gene has a significant effect on the increase of the body weight and carcass traits. This study has been carried out with the objective of polymorphism analysis in the exon 3 of myostatin in Karakul sheep breed, by PCR-RFLP method and its relationship with weight traits issues. Blood samples were individually randomly collected from 100 Karakul sheep in Karakul Sheep Breeding Station at Sarakhs. Genomic DNA was extracted by optimized clotted blood method. With a pair of predesigned primers, the 337 bp fragment of the exon 3 of myostatin gene was amplified. The PCR product was digested by HaeIII restriction enzyme to be used to detect genotypes. All samples in this locus were monomorph, with “mm” genotype. according to available reports and this results it is detected that the Iranian breeds have very low level of polymorphism, while the European breeds demonstrate higher level of mutated allel frequency. The lowness of this gene's frequency in Iranian breeds is of the genetic characteristic features of Iranian sheep breeds.

 
Keyword(s): DNA, KARAKUL, MYOSTATIN GENE, PCR-RFLP, POLYMORPHISM
 
References: 
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