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Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   SPRING 2013 , Volume 8 , Number 1 (32); Page(s) 99 To 110.
 
Paper: 

EVALUATION OF OXYGEN RADICALS EXCHANGE EFFECT ON EXPRESSION OF SOME ANTI OXIDANT GENES IN WHEAT

 
 
Author(s):  FAEZ P., NAVABPOUR S.*, ASGHARI A., RAMEZANPOUR S.
 
* GORGAN AGRICULTURE SCIENCE AND NATURAL RESOURCE UNIVERSITY
 
Abstract: 

To evaluate the silver nitrate as oxidative treatment and per-treatment of ascorbic acid on expression of catalase and super-oxide dismutase anti-oxidant genes in wheat, the experiment carried out in 2009 at research green house of GUASNR university. The experiment designed in factorial order by using Randomized Block with 4 replicates. Experiment treatments included three level of silver nitrate (0, 0.75, 1.5 mM), tow level of ascorbic acid (0, 20 mM) and tow wheat cultivars Falat and Tajan, sensitive and relatively resistance to oxidative stress respectively. Chemical treatment were applied by spray them on leaves at spikel appearance. Water spray used as control, for combined treatments the first one ascorbic acid was taken place on to plant followed 1 h later by silver nitrate. For all treatments leaf samples were taken 10 and 48 h after the last spray. Chlorophyll a, b content, TBARM measurement, super-oxide and hydrogen peroxide level and super-oxide dismutase and catalase gene expression were assigned. The result showed silver nitrate spray significantly reduced chlorophyll content in compare with control. Applying ascorbic acid followed 1 h later by silver nitrate resumed chlorophyll content in compared with silver nitrate spray, indicating anti oxidant role of ascorbic acid. In contrast to chlorophyll changes, TBARM level has increased by silver nitrate spray and relatively recoverd by combined treatment (ascorbic acid + silver nitrate). This was almost same for super-oxide ion and hydrogen peroxide levels. In all treatments super-oxide dismutase gene expression was more than catalase especially in Tajan cultivar.

 
Keyword(s): ANTIOXIDANT GENES, ACTIVE OXYGEN SPECIES, CELLULAR OXIDATIVE LEVELS, HYDROGEN PEROXIDE, SUPEROXIDE ION
 
References: 
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