Paper Information

Journal:   JUNDISHAPUR JOURNAL OF NATURAL PHARMACEUTICAL PRODUCTS   NOVEMBER 2014 , Volume 9 , Number 4; Page(s) 0 To 0.
 
Paper: 

CROCIN EFFECTS ON HUMAN MYELOMA CELLS REGARDING INTRACELLULAR REDOX STATE, DNA FRAGMENTATION, AND APOPTOSIS OR NECROSIS PROFILE

 
 
Author(s):  REZAEE RAMIN, JAMI ALAHMADI KHADIJE, RIAHI ZANJANI BAMDAD, MAHMOUDI MAHMOUD, ABNOUS KHALIL, ZAMANI TAGHIZADEH RABE SHAHRZAD, TABASI NAFISEH, ZALI MARJAN, REZAEE MARJAN, AMIN BAHAREH, KARIMI GHOLAMREZA*
 
* MEDICAL TOXICOLOGY RESEARCH CENTER, SCHOOL OF PHARMACY, MASHHAD UNIVERSITY OF MEDICAL SCIENCES, MASHHAD, IR IRAN
 
Abstract: 

Background: Well-documented studies reported several pharmacological properties for crocin, the active compound of Crocus sativus, such as its antitumor, radical scavenging, antidepressant, and memory-enhancing effects.
Objectives: We aimed to evaluate the possible cytotoxic activity of crocin on B lymphocytes in human myeloma (U266 cell line) after 24- and 48-hour treatment.
Materials and Methods: For this purpose, cell viability was determined by the colorimetric MTT assay and cell death pattern was evaluated using Annexin V-FITC/propidium iodide (PI) apoptosis detection kit. ROS (reactive oxygen species) production and DNA fragmentation were assessed using 2?, 7?-dichlorofluorescein diacetate (DCFH-DA) kit and PI staining, respectively.
Results: The highest concentration of crocin significantly decreased ROS production after 48 hours of treatment. However, crocin had no effect on the expression level of HSP (Heat shock protein). Additionally, its administration caused a mild decline in cell viability and a mild increase in the population of DNA fragmented cells as well as apoptosis.
Conclusions: In our study, no prominent effect was seen; therefore, in order to have a better perspective of crocin activity against cancerous cell lines, further studies are highly recommended.

 
Keyword(s): APOPTOSIS, DNA FRAGMENTATION, NECROSIS, CROCUS
 
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