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Journal:   MODERN GENETICS JOURNAL (MGJ)   SPRING 2013 , Volume 8 , Number 1 (32); Page(s) 37 To 46.
 
Paper: 

Transformation Of Beta-Glucoronidase Gene To Plastid Of Lettuce Iranian Variety (Lactuca Sativa. L)

 
 
Author(s):  Ghasemi E., Honari H., Omidi M., Shahnejat Bushehri A.A., Jalali M., Alizadeh H.*
 
* UNIVERSITY COLLEGE OF AGRICULTURAL & NATURAL RESOURCES, UNIVERSITY OF TEHRAN, TEHRAN, KARAJ
 
Abstract: 
Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression and reducing environmental contamination risks. Here, the development of a specific plastid vector for lettuce, (Lactuca sativa L. TN-96-39), was described using of rbcl and accD as target fragments, Prrn promoter, psbA terminator and a gene cassette for antibiotic resistance. The species-specific vector for plastid transformation to Lactuca sativa, called pCL 96-39 (TN-96-39 (Iranian line) chloroplast plasmid), has been sequenced. Plastid transformation was optimized by biolistic bombardment. Transfomants were confirmed by PCR and also histochemical test for GUS protein production. Beta-glucoronidase production was confirmed by SDS-PAGE electrophoresis in transformants. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants.
 
Keyword(s): GUS GENE, IRANIAN LETTUCE, PLASTID, VECTOR
 
 
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APA: Copy

GHASEMI, E., & HONARI, H., & OMIDI, M., & SHAHNEJAT BUSHEHRI, A., & JALALI, M., & ALIZADEH, H. (2013). TRANSFORMATION OF BETA-GLUCORONIDASE GENE TO PLASTID OF LETTUCE IRANIAN VARIETY (LACTUCA SATIVA. L). MODERN GENETICS JOURNAL (MGJ), 8(1 (32)), 37-46. https://www.sid.ir/en/journal/ViewPaper.aspx?id=411013



Vancouver: Copy

GHASEMI E., HONARI H., OMIDI M., SHAHNEJAT BUSHEHRI A.A., JALALI M., ALIZADEH H.. TRANSFORMATION OF BETA-GLUCORONIDASE GENE TO PLASTID OF LETTUCE IRANIAN VARIETY (LACTUCA SATIVA. L). MODERN GENETICS JOURNAL (MGJ). 2013 [cited 2022August08];8(1 (32)):37-46. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=411013



IEEE: Copy

GHASEMI, E., HONARI, H., OMIDI, M., SHAHNEJAT BUSHEHRI, A., JALALI, M., ALIZADEH, H., 2013. TRANSFORMATION OF BETA-GLUCORONIDASE GENE TO PLASTID OF LETTUCE IRANIAN VARIETY (LACTUCA SATIVA. L). MODERN GENETICS JOURNAL (MGJ), [online] 8(1 (32)), pp.37-46. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=411013.



 
 
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