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Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   SPRING 2013 , Volume 8 , Number 1 (32); Page(s) 37 To 46.
 
Paper: 

TRANSFORMATION OF BETA-GLUCORONIDASE GENE TO PLASTID OF LETTUCE IRANIAN VARIETY (LACTUCA SATIVA. L)

 
 
Author(s):  GHASEMI E., HONARI H., OMIDI M., SHAHNEJAT BUSHEHRI A.A., JALALI M., ALIZADEH H.*
 
* UNIVERSITY COLLEGE OF AGRICULTURAL & NATURAL RESOURCES, UNIVERSITY OF TEHRAN, TEHRAN, KARAJ
 
Abstract: 

Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression and reducing environmental contamination risks. Here, the development of a specific plastid vector for lettuce, (Lactuca sativa L. TN-96-39), was described using of rbcl and accD as target fragments, Prrn promoter, psbA terminator and a gene cassette for antibiotic resistance. The species-specific vector for plastid transformation to Lactuca sativa, called pCL 96-39 (TN-96-39 (Iranian line) chloroplast plasmid), has been sequenced. Plastid transformation was optimized by biolistic bombardment. Transfomants were confirmed by PCR and also histochemical test for GUS protein production. Beta-glucoronidase production was confirmed by SDS-PAGE electrophoresis in transformants. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants.

 
Keyword(s): GUS GENE, IRANIAN LETTUCE, PLASTID, VECTOR
 
References: 
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