Golden grey mullet (Liza aurata) is a commercially valuable fish in southern coastal parts of the Caspian Sea. In this study, we have used six microsatellite locations to investigate the level of genetic variation of Liza aurata in Babolsar and Chalus in Mazandaran province. The results showed no conspicuous genetic variation in these two regions using Fst and AMOVA and a relatively high level of gene flow was found among populations. Genetic variations in Babolsar (mean number of alleles per locus, Na=12.833, mean effective number of alleles, Ne= 9.402, observed heterozygosity, Ho=0.756 and expected heterozygosity, He=0.887) and Chalus (Na=13.667, Ne=9.945, Ho=0.708 and He=0.882) were not statistically different.