Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   SUMMER 2013 , Volume 8 , Number 2 (33); Page(s) 199 To 206.
 
Paper: 

RELATIVE EXPRESSION ANALYSIS OF FOUR TERPENE SYNTHASE IN ARTEMISIA SPECIES

 
 
Author(s):  RANJBAR M.*, NAGHAVI M.R., ALIZADEH H., SOLTANLOO H., ZALI A., TAGHIZAD FARID R.
 
* COLLEGE OF AGRICULTURE AND NATURAL RESOURCES, UNIVERCITY OF TEHRAN
 
Abstract: 

The pharmaceutical properties of artemisia are partially attributed to their essential oils, containing a wide variety of active phytochemicals, such as monoterpenes, sesquiterpenes and triterpenes. Among important terpens are b-amyrin, b-caryophyllene, Linalool and b-Pinene that are used as anticancer, analgesic, antimicrobial, antioxidant and anti-inflammatory. In this study, we have examined the relative expression levels of four genes of b-amyrin synthase, b-caryophyllene synthase, Linalool Synthase, and b-Pinene Synthase in eight species of Artemisia by real-time PCR. We used 18S rRNA as a control gene. Maximum and minimum expression levels of b-amyrin synthase, b- caryophyllene synthase, Linalool Synthase and b-Pinene Synthase were observed related to A. annua at the flowering stage of A. scoparia (1.62) and the seedling stage of A. sieberi (-50.85), the flowering stage of A. campestris (56.28) and the flowering stage of A. vulgaris (-10.74), the budding stage of A. scoparia (15.86) and the budding stage of A. sieberi (-20), the budding stage of A. campestris (53.57) and the budding stage of A. diffusa (-23.49), respectively.

 
Keyword(s): ARTEMISIA, GENE EXPRESSION, REAL-TIME PCR, SESQUITERPENE
 
References: 
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