Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   SUMMER 2013 , Volume 8 , Number 2 (33); Page(s) 177 To 188.
 
Paper: 

THE COMPARISON OF GENETIC DIVERSITY OF ERWINIA AMYLOVORA STRAINS HRP, AMS AND DSP GENE CLUSTERS AND PEA29 PLASMID

 
 
Author(s):  RAHIDEH S., ABDOLLAHI H.*
 
* HORTICULTURAL RESEARCH DEPARTMENT, SEED AND PLANT IMPROVEMENT INSTITUTE
 
Abstract: 

The bacteria Erwinia amylovora is the causal agent of fire blight disease in Maloideae fruit trees. In order to the evaluation of pathogenic intensity relation with strains genetic characterizations, in this research genetic diversity of three gene clusters including hrp, ams and dsp, interfering the pathogenicity were compared using PCR-RFLP marker. Moreover, the diversity of bacterial plasmid pEA29 were evaluated with PEANT pair primers. The used strains were 17 strains from Iran's domestic hosts and Ea273 strain from America. After designing the specific primers on mentioned gene clusters and PCR reaction, products were digested with AluI, BglII, HhaI, MboI, PstI, SalI and TaqI enzymes. In addition 5 ams gene cluster PCR products in 5 strains with geographical spread were sequenced. The results didn't show any variation in binding site and band length of different gene cluster primers. Moreover digestion with restriction enzymes created identical restriction pattern in all strains. Also PCR products sequencing of ams gene cluster showed complete similarity that represents very high genomic conservity in this gene cluster. The diversity assessment of amplified region with PEANT primers in pEA29 plasmid confirmed the length and sequence differences of this region in different strains. It seems that conservity level of this plasmid is much lower than genome of this bacteria.

 
Keyword(s): FIRE BLIGHT DISEASE, GENETIC DIVERSITY, HRP,AMS AND DSP GENE CLUSTERS
 
References: 
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