Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   SUMMER 2013 , Volume 8 , Number 2 (33); Page(s) 169 To 176.
 
Paper: 

INVESTIGATION OF GENETIC DIVERSITY AMONG PISTACHIO VERA IN THE KHORASAN BY USING MOLECULAR MARKER

 
 
Author(s):  TAYEFEH ALIAKBARKHANY S.*, TALAIE A.R., FATAHI MOGHADAM M.R.
 
* UNIVERSITY OF TEHRAN, KARAJ, IRAN
 
Abstract: 

Accurate and positive identification of plant genotypes, especially those of fruit trees, is very important in implementing breeding programs and in preserving germ plasms. Evaluation of genetic diversity is essential in identifying different genes, estimation of genetic distances between and within individuals and populations and determination of heterosysis. In spite of reach pistachio genetic resources in Iran, a few studies have been conducted on this genus. In the present study, genetic diversity of 38 male and female pistachio genotypes in Faizabad region of Khorasan province were investigated using RAPD markers. The fifteen primers used produced 115 polymorphic loci. The highest number of loci (12) were for primer. The average of polymorphic loci percentage was 92.83. The resolving power for primers ranged from 1.36 to 7.89, averaging. The lowest genetic similarity (0.18) was between genotypes ‘Garme Siah’ and ‘Sefid Sabuni1’ and the highest of that (0.70) detected between ‘Badami Sefid3’ and ‘Genotype7’. Cluster analysis based on Dice similarity coefficients and UPGMA method divided the genotypes into 8 at similarity level of 0.41.The results showed the exictence of the high genetic diversity in the studied genotypes and a good performance of the RAPD markers for study of the genetic diversity of pistachio.

 
Keyword(s): DICE SIMILARITY COEFFICIENT, GENETIC VARIABILITY, PISTACHIO, RAPD
 
References: 
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